摘要
目的利用曲古霉素A(TSA)处理人胃癌细胞系BGC-803后观察细胞增殖情况并研究了凋亡相关基因表达量的变化。方法本研究通过TSA处理胃癌细胞系BGC-803细胞后,利用MTT、Real-time PCR、Western blot等试验方法,采用SPSS 17.0统计软件进行数据分析。结果 75 ng/m L的TSA处理48 h相较其他试验组,可显著抑制胃癌细胞系BGC-803细胞增殖(P<0.05),且其抑制作用具有剂量、时间依赖性;使促凋亡蛋白BAX、Caspase-3的mRNA、蛋白表达量升高,从而发挥促进细胞凋亡的作用。结论 TSA诱导BGC-803调亡是通过Caspase依赖途径进行的,且随着时间和剂量的增加,TSA对胃癌细胞的抑制效果越来越显著。
Objective To observe the cell multiplication status after dealing with human gastric cancer cell line BGC-803 by means of Trichostatin A（TSA）,and to study the changes of apoptosis related gene expression quantity. Methods After dealing with human gastric cancer cell line BGC-803 by means of TSA, the study adopted test methods of MTT,Real-time PCR,and Western blot and SPSS 17.0 statistical software to conduct data analysis.Results Compared with other experimental groups, 48 h of 75 ng/mL TSA treatment could significantly inhibit the cell multiplication of gastric cancer cell line BGC-803（P〈0.05）, and the inhibitory effect bears dose and time dependence.It promoted pro-apoptotic protein BA.X, mRNA of Caspase-3, and protein expression, which consequently exerted the function of promoting apoptosis.Conclusion TSA induced gastric cancer cell line BGC-803 apoptosis is carried out through Caspase-dependent pathway, and with the increase of time and dose, TSA shows increasingly significant inhibitory effect on gastric cancer cells.
出处
《中国疗养医学》
2016年第10期1016-1018,共3页
Chinese Journal of Convalescent Medicine
