弥漫性大B细胞淋巴瘤的分子遗传学研究

Study of molecular genetic on diffuse large B -cell lymphoma

目的：探讨弥漫性大 B 细胞淋巴瘤（DLBCL）的分子遗传学特征以及免疫表型分型的特点，为进一步研究提供依据。方法收集92例弥漫性大 B 细胞淋巴瘤组织标本，通过 Hans 分型法进行分型，包括中心 B 细胞样（GCB）亚型及非 GCB 亚型，通过荧光原位杂交方法对 bcl-2、bcl-6基因进行检测，并对亚型分型与基因表达的关系进行分析。结果 GCB 亚型28例，构成比为30．43％，非 GCB 型64例，构成比为69．57％。bcl-2基因异常较多发生于 GCB 亚型的淋巴瘤中，差异有统计学意义（χ^2＝38．39，P ＜0．05），而 bcl-6基因异常在 GCB 亚型及非 GCB 亚型中的分布差异无统计学意义（χ^2＝0．56，P ＞0．05）。bcl-2基因易位与 bcl-6基因易位无相关性（r ＝0．095，P ＞0．05），bcl-2基因扩增与 bcl-6基因易位显著相关（r ＝0．750，P ＜0．01）。免疫表型亚型分类与发病部位无相关性（r ＝0．174，P ＞0．05），bcl-2基因表达、bcl-6基因表达与发病部位也无相关性（r ＝0．205，r ＝0．188，均 P ＞0．05）。结论 DLBCL 患者的 GCB 亚型中多出现 bcl-2基因异常，但 bcl-2与bcl-6基因表达的关系尚不明确，且发病部位与分子遗传学可能并无关联。

Objective To study the molecular genetic characteristics and immunophenotype of diffuse large B -cell lymphoma （DLBCL）,thus to provide evidence in further research.Methods 92 patients with DLBCL were selected.Immunophenotype was classified by Hans method,bcl -2 and bcl -6 were detected by fluorescence in situ hybridization（FISH）.The relationship between immunophenotype and genes expression was analyzed by SPSS 19.0. Results There were GCB with 28 cases（30.43%）and non GCB with 64 cases（69.57%）.Bcl -2 gene abnormality was more found in GCB（χ^2 =38.39,P 〈0.05）,there was no difference of bcl -6 abnormality between GCB and non GCB（χ^2 =0.56,P 〉0.05）.There was no correlation between bcl -2 translocation and bcl -6 translocation（r =0.095,P 〉0.05）,but there was significant correlation between bcl -2 amplification and bcl -6 translocation（r =0.750,P 〈0.01）.There were no correlations between the location and immunophenotype,bcl -2,bcl -6（r =0.174, r =0.205,r =0.188,all P 〉0.05 ）.Conclusion Bcl -2 gene abnormality was more found in GCB,but the relationship between bcl -2 and bcl -6 is not clear.There may be no correlation between location and molecular genetic.