犏牛、牦牛睾丸组织中Ghrelin与GHSR基因mRNA表达水平研究

Expression level of Ghrelin and GHSR mRNA in testis of cattle-yak and yak

对犏牛、牦牛睾丸组织中生长素(Ghrelin)与生长激素促分泌素受体(GHSR)基因mRNA表达水平进行研究。运用荧光定量PCR方法,检测Ghrelin、GHSR基因在犏牛、牦牛睾丸组织中m RNA的表达水平差异,结果显示:在犏牛、牦牛睾丸组织中,Ghrelin基因m RNA相对表达量犏牛显著高于牦牛(分别为0.8177±0.0225、0.4656±0.0222,P<0.05);GHSR基因mRNA相对表达量也是犏牛显著高于牦牛(分别为0.8622±0.0347、0.4722±0.0761,P<0.05)。相对于牦牛睾丸组织,犏牛睾丸组织中Ghrelin与GHSR基因m RNA均高表达可能是犏牛雄性不育的原因之一。

The expression level of Ghrelin and growth hormone secretagogue receptor（GHSR） gene m RNA tested respectively in testis of cattle-yak and yak by using fluorescence quantitative polymerase chain reaction （PCR）. The results showed that Ghrelin m RNA expression level in cattle-yak testis was significantly higher than that in yak （0.8177±0.0225,0.4656±0.0222,P〈0.05）. Similarly,GHSR m RNA expression level in cattleyak testis was significantly higher than that in yak（0.8622±0.0347,0.4722±0.0761,P〈0.05）. These results indicated that both Ghrelin and GHSR m RNA expression level in cattle-yak testis was significantly higher than that in yak,which was likely to be one of the reasons for male infertility of cattle-yak.