广东省猪圆环病毒2型基因的克隆与序列分析

CLONGING AND SEQUENCING OF PORCINE CIROVIRUS TYPE 2 IN GUANGDONG

根据Gen Bank已发表的猪圆环病毒2型（Porcine circovirus type 2,PCV2）全基因组序列,设计1对特异性引物,对广东省不同地区规模化猪场采集的临床诊断为断奶仔猪多系统消耗综合征（Postweaning multisystemic wasting syndrome,PMWS）的组织病料进行了PCV2基因克隆和序列分析。结果表明,采集的PMWS病料均可扩增出PCV2完整基因序列,与Gen Bank已发表的PCV2参考毒株序列核苷酸同源性达93.7%-99.8%,亲缘关系密切。其中10个PCV2基因全长1767 bp,属于PCV2b亚型,1个PCV2基因全长1768 bp,属于PCV2a亚型。

A pair of specific primers were designed according to the whole genomic sequence of porcine circovirus type2（ PCV2 ） published on GenBank. The suspicious samples were collected from large scale pig farms in different areas of Guangdong province and examined in PCR and resulting amplicons were cloned and sequenced. The results showed that nucleotide sequences of 11 PCV2 isolates were stable and their similarities were 93.7% to 99.8% with the reference strain published in the GenBank. The complete sequences of 10 PCV2 isolates were1767 bp and belonged to PCV2b subtype. However, the complete sequence of one PCV2 isolate was 1768 bp and belonged to PCV2a subtype.