蝶豆的组织培养与快繁技术研究

Study on Tissue Culture and Rapid Propagation of Clitoriaternatea L.

通过蝶豆种子无菌萌发,筛选出各培养阶段的适宜培养基,为建立蝶豆的组织培养与快繁技术提供参考。结果表明:采用75%酒精表面灭菌30 s结合0.1%的HgCl2表面灭菌10 min的方法,蝶豆的种子无菌萌发时污染率为0,发芽率为36.67%;下胚轴、上胚轴、子叶的愈伤诱导率为100.00%,愈伤分化率分别为82.11%、20.00%、0;愈伤分化的最佳培养基为MS+6-BA0.10mg/L+NAA 0.02 mg/L;上胚轴和下胚轴不能直接诱导出不定芽,子叶可以直接诱导出不定芽;不定芽增殖的适宜培养基为MS+6-BA 1.00 mg/L+IBA 0.50 mg/L;生根适宜培养基为MS+IBA 0.30 mg/L或者1/2 MS+IBA 0.30 mg/L。

The suitable medium for varied stages was screened out by axenic germination of Clitoriatematea L. to provide references for tissue culture and rapid regeneration of Clitoriaternatea L. The results showed that： by the method of 75 % ethanol for 30 s ＋0.1% HgCl2 for 10 rain, the contamination rate was 0 and the germination rate was 36.67 % when the seeds were aseptic budding. The callus induction rate of hypocotyls, epicotyls, and cotyledon was 100.00 % and the rate of callus differentiation was 82.11%, 20.00 % and 0, respectively. The optimizing medium for callus differentiation was MS ＋6-BA 0. 10 mg/L ＋ NAA 0.02 mg/L. Adventitious buds cannot be induced directly from epicotyl and hypocotyl, but can be induced directly from cotyledon. The optimizing medium for adventitious bud multiplication is MS ＋ 6-BA 1.00 mg/L ＋ IBA 0.50 mg/L. The most suitable medium for adventitious root is MS ＋ IBA0.30 mg/L or 1/2M5 ＋ IBA0.30 mg/L.