枯草芽孢杆菌YB5对菜豆根腐病菌的抑菌机制测定及应用

Application and Antagonistic Mechanism of Bacillus subtilis YB5 Gainst Fusarium solani Causing Green Bean Root Rot

腐皮镰孢引起的菜豆根腐病是一种世界性病害,在菜豆生产中危害严重。为对其有效的生物防治,针对菜豆根腐病利用枯草芽孢杆菌进行了拮抗机制及应用的研究。结果表明:YB5能有效抑制菌丝生长、孢子产生和萌发。YB5的无菌滤液对高温敏感。YB5菌液对种子发芽没有抑制作用,且能促进根系的发育。YB5菌液及无菌滤液对菜豆腐皮镰孢根腐病的盆栽均具有较好的防效,YB5菌液防效可达94.6%。

Fusariurn root rot of bean caused by Fusarium solani f. sp. phaseoli is an important disease of this crop worldwide and has economic impact in green bean producing areas. In order to biological control,Bacillus subtilis YB5 was a potential bacterial hiocontrol agent against F. solani causing green bean root rot. And application and antagonistic mechanism of YB5 against F. solani was tested. The results showed that secondary metabolite production by YB5 could effectively inhibit mycelial growth, spores bearing and germination of F. solani and was sensitive to high-temperature. And YB5 bacterial suspension had no effect on green bean seeds germination and could encourage root development. YB5 secondary metabolite and bacterial suspension could effectively protect green bean against root rot caused by F. solani. And its control effect was up to 94.6% by potted plant experiments.