摘要
为了探究大球盖菇菌丝体液体发酵的最佳培养基配方及培养条件,研究了碳氮源不同添加量及不同培养条件对其菌丝生长的影响。结果表明:大球盖菇菌丝体液体发酵的最佳培养基配方为可溶性淀粉2%,葡萄糖1%,米糠3%,KH_2PO_40.20%;最适培养条件为pH6.5,接种量8%,摇床转速130r·min^(-1),培养周期为7d。
In order to explore the optimum mdeium formula and fermentation conditions on the submerged-culture of Stropharia rugoso-annulata mycelium, the effects of different additive amount of C-sources and Nsources and culture conditions on yield of Stropharia rugoso-annulata mycelium were studied. The results showed that the optimum culture medium formula of incubation was determined： soluble stach 2%, glucose 1%, rice bran 3%, KH2PO4 0.20% ;The optimum culture condition was dertermined： pH6.5, inoculum 8%, rotary speed 130 r·min^-1 ,fermentation time for 7 d.
出处
《黑龙江农业科学》
2016年第8期114-117,共4页
Heilongjiang Agricultural Sciences
关键词
大球盖菇菌丝体
液体发酵
培养条件
Stropharia rugoso-annulata mycelium
liquid fermentation
culture condition
