血浆miR-92a与骨肉瘤预后相关性研究

Correlation of miR-92a in plasma with prognosis in osteosarcoma

背景与目的:骨肉瘤是儿童和年轻成人最常见的原发性恶性骨肿瘤,目前尚无高特异度、高灵敏度的预后预测指标。该研究旨在探讨血浆mi R-92a表达水平与骨肉瘤临床病理及预后的关系。方法:收集30例健康体检者和45例骨肉瘤患者术前及术后血浆,通过实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,RTFQ-PCR)检测血浆mi R-92a水平。以术前mi R-92a≥5为界限,将骨肉瘤患者分为高表达组和低表达组。结果:骨肉瘤患者血浆mi R-92a水平显著高于正常体检人群,患者术前mi R-92a水平远高于术后。患者术前mi R-92a表达水平与患者的性别、年龄、肿瘤部位、肿瘤大小和组织学类型等无关,而与Enneking分期、肿瘤坏死率和肺转移相关。Kaplan-Meier分析高表达mi R-92a的骨肉瘤预后差(P=0.035)。结论:骨肉瘤患者血浆mi R-92a表达水平与生存率相关,可能是骨肉瘤一项有价值的预后指标。

Background and purpose： Osteosarcoma is the most common primary malignant bone tumor in the children and young adults. Until now, there is no prognostic indicator with high specificity and high sensitivity. This study investigated the relationship between miR-92a level in palsma and clinical pathological characteristics, as well as the prognosis in osteosarcoma. Methods： The plasma from 30 healthy volunteers who underwent physical examination and from 45 cases of osteosarcoma before and after operation were collected. The miR-92a level was detected by real- time fluorescent quantitative polymerase chain reaction （RTFQ-PCR） method. According to the relative expression 1〉 5 of miR-92a, the osteosarcoma patients were divided into 2 groups： miR-92a high expression group and low expression group. Results： The miR-92a level in palsma from osteosarcoma is significantly higer than that from healthy volunteers. Before surgery, the miR-92a level was markedly higher than after surgery. There was no correlation between the miR- 92a level and the gender, age, tumor location, tumor size and histological type. However, significant correlation was found with Enneking grade, tumor cell necrosis rate and lung metastasis. Compared with the low-expression group, high miR-92a expression was associated with a significantly shorter overall survival using Kaplan-Meier analysis （P=0.035）. Conclusion： The results suggest a significant relationship between miR-92a and survival ratio, miR-92a expression level may be a useful prognostic indicator in osteosarcoma.