莲瓣兰大雪素FT基因克隆和系统发育分析

Isolation and Phylogenetic Analysis of a FT Gene fromCymbidium tortisepalum

控制植物开花的途径有光周期现象、GA、春化途径、自主途径,在光周期途径中,CO基因促进开花通过直接上调FT和SOC1基因表达,FT(FLOWERING LOCUS T)是光周期途径植物开花时间决定关键基因,并认为FT基因表达产物可能就是长期追寻的开花刺激物质,这种开花刺激物质经过叶片到茎尖的长距离运输,最终引起茎顶端花器官分化的起始。本研究以莲瓣兰大雪素作为实验材料,用RACE方法快速地克隆全长cDNA,生物信息学分析全长cDNA为662bp,含有编码框和3′UTR,具有完整的开放阅读框(ORF)522bp,编码173氨基酸的蛋白质。通过系统发育分析获得一个FT同源基因。本研究为进一步对这个基因的功能研究奠定了基础。

There are photoperiod,GA,vernalization,and independent pathways to control plant flowering.CO gene promotes flowering through regulating FT（FLOWERING LOCUS T）and SOC1（SUPRESSOR OF OVEREXPRESSION OF CONSTANS1）genes expression directly in the photoperiod pathway.FTis considered that it is a key gene to regulate flowering time in pathway,and its expression productions are flowering stimulating substances for a long time.The kind of flowering stimulating substances transport from leaves to the top of stems,and ultimately causes flowering.Using rapid amplification of cDNA ends（RACE）,FT gene was cloned quickly.Bioinformatics analysis showed that the full length was 662 bp,with the open reading frame（ORF）of this gene was 522 bp,encoding 173 amino acids.We obtained the FT gene fromCymbidium tortisepalum with phylogenetic analysis.It is the basic for further study of this gene function.