期刊文献+

重组毕赤酵母(Pichia pastoris)高产Lunasin的发酵工艺优化 被引量:3

Optimization for High Production Fermentation of Lunasin from Recombinant Pichia pastoris
原文传递
导出
摘要 Lunasin是一个最初从大豆中分离,具有43个氨基酸的多肽,具有抗高血压,抗氧化活性,预防癌症,抗炎和降胆固醇的活性。为了实现Lunasin的高效生产,对重组毕赤酵母GS115 LN诱导阶段甲醇补料策略(溶氧反馈补料,指数-恒速补料)以及诱导温度进行了优化,并且在此基础上对双碳源(山梨醇、甘露醇)和甲醇混合补料策略以及复合氮源与甲醇混合补料策略进行了优化。研究表明,最优补料策略以及诱导温度分别为指数-恒速补料、24℃,最终在流加1%大豆蛋白胨以及0.02%天冬氨酸条件下,Lunasin表达量最高,达到3.27 g/L,是单一甲醇诱导的1.27倍。 Lunasin is a 43 amino acid polypeptide originally isolated from soybean with bioactive properties such as antihypertensive, antioxidant activity, cancer prevention and therapy, anti-inflammation, hypocholesterolemic activity, anti obesity and immunomodulation. In order to achieve efficient production of Lunasin, the methanol fed methods (DO feedback fedding and index - constant speed feeding) and induction temperature of recombinant Pichia pastoris GSl15 LN were investigated. Furthermore, the feeding strategies of mixed carbon sources during induction phase were investigated. The results show that the best feeding strategies and induced temperature were index-constant speed feeding, 24 ℃. Consequently, the highest expression of Lunasin was 3.27g / L by feeding 1% soy peptone and 0. 02% aspartic acid,which was 1.27 times higher than the single methanol induction process.
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2016年第8期73-79,共7页 China Biotechnology
基金 科技部支撑计划资助项目(2013BAD10B02)
关键词 Lunasin 重组毕赤酵母 高密度发酵 工艺优化 Lunasin Recombinant Pichia pastoris High density fermentation Process optimization
  • 相关文献

参考文献5

二级参考文献64

  • 1王洪海,高卜渝,陈佩丽,董灵,李育阳.酵母表达基因工程产物不均一性分析及其对策[J].中国科学(B辑),1994,24(12):1270-1274. 被引量:12
  • 2史琪琪,郝玉有,吴康华,储炬,庄英萍,张嗣良.诱导相温度对毕赤酵母表达重组人复合α干扰素聚合的影响[J].生物工程学报,2006,22(2):311-315. 被引量:12
  • 3张惠展著.基因工程概论[M].上海:华东理工大学出版社,2000..
  • 4Galvez AF, Chen N, Macasieb J, et al. Chem preventive property of a soybean peptide (Lunasin) that binds to deacetylated histones and inhibits acetylation[J]. Cancer Res, 2001, 61:7473 - 7478.
  • 5Park JH, Jeong HJ, Lumen BO. In vitro degestibility of the cancer-preventive soy peptides Lunasin and BBI [J]. Agric Food Chem, 2007, 55:10703 - 10706.
  • 6Park JH, Jeong H J, Lumen BO. Contents and bioactivities of Lunasin, Bowman-Birk Inhibitor, and Iso?avones in soybean seed [J]. Agric Food Chem, 2005, 53:7686 - 7690.
  • 7Jeong H J, Larn YI, and Lumen BO. Barley Lunasin suppresses ras-induced colony formation and inhibits core histone acetylation in mammalian cells[J]. Agric Food Chem, 2002, 50:5903 - 5908.
  • 8Jeong HJ, Jeong JB, Kim DS, et al. The cancer preventive peptide Lunasin fom wheat inhibits core histone acetylaion[J]. Cancer Letters, 2007, 255:42 - 48.
  • 9Kennedy AR. The evidence for soybean products as cancer preventive agents[J]. Nutr, 1995, 125:733 - 743.
  • 10Shahidi F, Zhong Y. Bioactive peptides [J]. Aoac International, 2008, 91 (4): 914 - 931.

共引文献37

同被引文献31

引证文献3

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部