黄芩素通过抗氧化应激延长果蝇寿命的机制

Baicalein prolongs the lifespan of Drosophila melanogaster through antioxidation activity

为了探究黄芩素是否具有抗衰老作用,本研究以果蝇为模式生物,考察在自然衰老模型、过氧化氢损伤模型和百草枯损伤模型中黄芩素对雌性果蝇寿命的影响。在此基础上,采用生物信息学方法预测黄芩素发挥抗衰老活性可能作用的靶点,并对靶点进行通路富集,选择影响较大的氧化应激通路进行实验验证。结果表明,0.04和0.2 mg·m L-1黄芩素均能延长自然衰老模型中果蝇的平均寿命和最高寿命,并可有效减弱由过氧化氢和百草枯引起的果蝇寿命缩短。靶点预测结果显示黄芩素可能作用于31个衰老相关靶点,并能显著影响氧化应激通路,进一步实验结果表明黄芩素可能通过降低果蝇体内活性氧(reactive oxygen species,ROS)、丙二醛(malondialdehyde,MDA)、氧化型谷胱甘肽(oxidized glutathione,GSSG)含量,从而减轻氧化应激水平,延缓衰老。

In order to explore the anti-aging effect of baicalein, female Drosophila melanogaster as a model organism was used to study the effects of baicalein on natural aging model and aging models induced by hydrogen peroxide （H202） and paraquat. The bioinformatics approach was used to predict the possible target for the anti-aging activity of baicalein, and the target pathways were identified. The oxidative stress pathway was a focus in experiment. Baicalein at concentrations of 0.04 mg .mL-1 and 0.2 mg-mL-1 extended the mean and maximum lifespans in the natural aging model, and effectively reduced the damages of oxidative stress by H202 and paraquat. 31 senescence-related targets together with the oxidative stress pathway were modulated by baicalein. The experiments revealed that baicalein might delay aging process through attenuation of the oxidative stress response by decreasing the reactive oxygen species （ROS）, malondialdehyde （MDA） and oxidized glutathione （GSSG） in Drosophila melanoeaster.