响应面优化朝鲜淫羊藿多糖提取条件及其肿瘤免疫活性研究

Response surface analysis for the optimization of extraction condition for polysaccharides from Epimedium polysaccharides and studies on its tumor immune activities

本文利用Box-Benhnken中心组合试验和响应面分析法,旨在提高朝鲜淫羊藿多糖的提取效率,并建立C57BL6小鼠Lewis肺癌模型,对所得多糖进行肿瘤免疫活性的初探。以多糖得率为指标,通过单因素实验对提取时间、温度和料液比进行考察,对试验数据进行二项式拟合,得到提取多糖的最佳条件:温度为87℃,时间为3.84 h,料液比为1∶16.33。在此条件下多糖得率预测值为1.045%,验证值1.023%,相对误差2.15%。该模型拟合度高,操作方便可行,为朝鲜淫羊藿多糖的提取提供了参考。动物实验表明,所得朝鲜淫羊藿多糖可以维持荷瘤机体免疫功能,提高正向免疫因子水平,调节负向免疫因素,具有明显体内抗肿瘤活性。

To enhance the efficiency of the extraction for Epimedium koreanum Nakai polysaccharides, we conducted the Box-Benhnken experiment and examined the response surface. C57 BL6 mice were used as Lewis-bearing mice model in the study of the polysaccharides in the regulation of tumor immunity. In the first place, different factors including extraction time, temperature, and ratio were evaluated in the yield of polysaccharides. The second order polynomial equation was formed to fit the experimental data. The optimal condition was temperature 87 ℃, extraction time 3.84 h, and ratio of solution and material 1 ： 16.33 to get polysaccharides from Epimediurn koreanum Nakai. Under this condition, model-predicted and experimentally measured values of polysaccharide yield were 1.045% and 1.023%, respectively, with a relative error between them at 2.15%. The extraction method is reliable, simple and applicable to the extraction of Epimedium poly- saccharides. In addition, this data suggests that Epimediurn polysaccharides have obvious immune-regulatory activities in the tumor-bearing mice through increasing the immune-enhancing cytokines to override the immune-suppressing factors.