卵巢癌组织细胞黏附分子1的表达变化及意义

Expression of CADM1 and its clinical significance in human ovarian carcinoma

目的探讨细胞黏附分子1（CADM1）在卵巢癌组织中的表达变化及其临床意义。方法收集卵巢癌患者59例,取其癌组织及癌旁正常组织,采用qRT-PCR、Western blotting和免疫组化法分别检测CADM1 mRNA和蛋白在卵巢癌及对应癌旁正常组织中的表达。χ^2检验及Kaplan-Meier生存曲线分析CADM1的表达水平与卵巢癌患者临床病理参数、预后的相关性。结果卵巢癌组织中CADM1 mRNA和蛋白表达水平分别为0.28±0.12、1.32±0.23,癌旁正常组织分别为1.52±0.14、2.93±0.42,癌组织与癌旁正常组织比较,P均〈0.05。癌旁正常组织中CADM1阳性细胞百分比（90%±5%）显著高于癌组织（10%±2%）（P〈0.05）。CADM1表达水平与卵巢癌患者FIGO分期、远处转移及淋巴结转移显著相关（P均〈0.05）。CADM1低表达（mRNA表达量〈0.57）患者中位生存期显著低于CADM1高表达患者（13.2比24.5个月,P〈0.05）。结论卵巢癌组织中CADM1呈低表达状态,低水平的CADM1促进卵巢癌的发生发展,且与其不良预后相关。

Objective To investigate the expression changes of cell adhesion molecule-1（ CADM1） in human ovarian carcinoma and its clinical significance. Methods A total of 59 case of ovarian carcinoma tissues and matched tumoradjacent tissues were collected. The mRNA and protein expression of CADM1 in the ovarian carcinoma tissues and matched tumor-adjacent tissues was examined by qRT-PCR,immunohistochemistry and Western blotting. The Chi-square test and Kaplan-Meier analysis was used to evaluate the correlation between CADM1 expression and clinicopathological characteristics or overall survival. Results The expression of CADM1 mRNA and protein in the ovarian carcinoma tissues was 0. 28± 0. 12 and 1. 32 ± 0. 23,respectively,1. 52 ± 0. 14 and 2. 93 ± 0. 42 in the matched tumor-adjacent tissues,and significant difference was found between these two kinds of tissues（ all P〈0. 05）. The percentage of positive cells in the adjacent tumor tissues（ 90% ± 5%） was significantly higher than that of the cancer tissues（ 10% ± 2%）,P〈0. 05. The expression of CADM1 was significantly associated with the FIGO stage,nodal metastasis and distant metastasis（ all P〈0. 05）. The median survival time of patients with low expression of CADM1（ mRNA expression 0. 57） was higher than that of patients with high expression（ 13. 2 vs. 24. 5 months,P〈0. 05）. Conclusion CADM1 is low expressed in the human ovarian cancer tissues. The low expression of CADM1 promotes the occurrence and development of ovarian cancer,and is related to its poor prognosis.