摘要
目的探讨氯沙坦钾联合环磷酰胺在治疗2型糖尿病肾病大鼠时对转化生长因子β1(TGF-β1)、CD68和单核细胞趋化因子蛋白-1(MCP-1)表达的影响。方法选择45只雄性健康SD大鼠,按随机数字表法分为正常对照组、2型糖尿病肾病模型组和氯沙坦钾联合环磷酰胺治疗组大鼠各15只。造模成功后,观察三组大鼠的血生化指标和肾脏病理学改变等,并采用免疫组化染色比较其TGF-β1、CD68和MCP-1的表达情况。结果与正常对照组相比,模型组和治疗组的体质量较低,在尿蛋白、血糖、甘油三酯、胆固醇和肌酐等血生化指标以及TGF-β1、CD68和MCP-1表达方面均显著高于对照组,且差异均有统计学意义(P<0.05);治疗组与模型组相比,甘油三酯、肌酐以及TGF-β1、CD68和MCP-1表达均较低(P<0.05)。结论氯沙坦钾联合环磷酰胺在治疗2型糖尿病肾病大鼠时,可以通过降低肾组织中TGF-β1、CD68和MCP-1的表达水平来降低炎细胞浸润和免疫反应程度,从而延缓糖尿病肾病病情的进展,但是否能用于临床有待于进一步研究。
Objective To investigate the effects of the expressions of TGF-β1, CD68 and MCP-1 by united in Losartan Potassium and CTX therapy in the type 2 diabetic nephropathy renal tissue. Methods Total of 45 SD male rats were randomized into three groups which were the normal control group, type 2 diabetic nephropathy group and Losartan Potassium combined with CTX treatment group （each group was 15 SD）. The serum biochemical parame- ters and renal pathologic featuresin of three, groups were observed. The expressions of TGF-β1, CD68 and MCP-1 in the renal tissue were detected by immunohistochemistry staining. Results The body weight of the rats in the model group and treatment group was significantly less than control group （P〈0.05）. There were statistical significantly differences in the urinary protein, blood glucose, triglyceride, creatinine, cholesterol and the expression of TGF-β1, CD68 and MCP-1 between the observation and control groups （P〈0.05）. And there were statistical significantly differences in triglyceride, creatinineand the expression of TGF-β1, CD68 and MCP-1 between the model group and treatment group （P〈0.05）. Conclusion Losartan Potassium and CTX protects the kidney of diabetic nephropathy rats through inhibiting the expression of TGF-β1, CD68 and MCP-1 in the kidney and restraining macrophage infil- tration and the degree and extent of immunoreaction, however, whether it can be used in the clinic that need future research.
出处
《贵州医药》
CAS
2016年第7期689-691,共3页
Guizhou Medical Journal
