ASE-UPLC法同时测定杜仲制剂中3种成分

Simultaneous Determination of Three Components in Preparations Containing Eucommia Ulmoides by a Method of ASE-UPLC

建立ASE-UPLC(快速溶剂萃取仪-超高效液相色谱)法同时测定不同杜仲制剂中京尼平苷酸(GPA)、绿原酸(CA)、松脂醇二葡萄糖苷(PDG)3种指标成分含量的方法.采用70%甲醇水,温度40℃,压力103.4MPa的ASE法提取;色谱柱Acquity UPLCBEHC18(100mm×2.1mm,1.7μm);乙腈(A)-0.1%甲酸水(B)为流动相;流速0.15m L/min;洗脱梯度:0~15min,70%B^90%B;检测波长237nm;柱温20℃.结果显示3种被测成分分别在选定的范围内线性关系良好;精密度、重复性良好,相对标准偏差(RSD)分别均小于3.0%;平均加标回收率在95.84%~102.69%,RSD均小于3.0%.表明所建立的方法快捷、准确、重复性好,可用于杜仲制剂中主要成分的含量测定.

An ASE-UPLC method for simultaneous determination of three components including geniposidic acid,chlorogenic acid and pinoresinol diglucoside in the different preparations containing Eucommia ulmoides was established.The ASE extraction was conducted with 70% MeOH-H 2 O as solvent at 40 ℃ and 103.4 MPa, and UPLC analysis was performed on an Acquity UPLC BEH C18 column （100 nm×2.1 mm,1.7 μm）with acetonitrile （A）-0.1% formic acid （B）as mobile phases for gradient elution （0 ~1 5 min,70% B^90% B）at the flow rate of 0.1 5 mL/min.The detection wavelength was 237 nm and the column temperature was 20 ℃. The results showed that three components were separated completely and showed good linearity,precision and repeatability （RSD ＆lt;3.0% respectively）.Recoveries were between 95.84% ~ 102.69% （RSD ＆lt; 3.0%）.The data suggested the established method was rapid,accurate,good reproducible and could be applied to simultane-ously determine main components in the preparations containing Eucommia ulmoides .