番茄ty-5和Mi-1基因多重PCR体系的建立

Identification of ty-5 gene and Mi-1 gene by multiplex PCR in tomato

为了用分子标记辅助选择法进行番茄育种时能快速鉴定抗番茄黄化曲叶病毒病基因ty-5和抗根结线虫病基因Mi-1,利用番茄材料1227(含有ty-5基因)、VFNT(含有Mi-1基因)和杂交获得的F2分离群体,通过分析扩增片段多态性差异,重新设计分子标记,建立了ty-5和Mi-1基因的多重PCR反应体系。通过对这2个基因的分离群体进行基因型和抗病性鉴定,证实标记检测结果与田间接种鉴定结果吻合率为95.0%,可以将该PCR体系用于对这2个基因的同时鉴定。

To realize the rapid identification of gene ty-5 resistant to tomato yellow leaf curl virus( TYLCV) and gene Mi-1 resistant to root-knot nematode( RKN) disease for tomato breeding through marker-assisted selection,a multiplex PCR reaction system was developed with tomato lines 1227( with ty-5 gene),VFNT( with Mi-1 gene) and segregation population F2 by analyzing the polymorphism of amplified fragment with redesigned molecular markers. The identification of the genotype and disease resistance in F2 population by multiplex PCR detection was 95% similar to that by field inoculation evaluation. The multiplex PCR system developed in this study is capable of simutaneous identification of ty-5 and Mi-1 in tomato.