摘要
为了用分子标记辅助选择法进行番茄育种时能快速鉴定抗番茄黄化曲叶病毒病基因ty-5和抗根结线虫病基因Mi-1,利用番茄材料1227(含有ty-5基因)、VFNT(含有Mi-1基因)和杂交获得的F2分离群体,通过分析扩增片段多态性差异,重新设计分子标记,建立了ty-5和Mi-1基因的多重PCR反应体系。通过对这2个基因的分离群体进行基因型和抗病性鉴定,证实标记检测结果与田间接种鉴定结果吻合率为95.0%,可以将该PCR体系用于对这2个基因的同时鉴定。
To realize the rapid identification of gene ty-5 resistant to tomato yellow leaf curl virus( TYLCV) and gene Mi-1 resistant to root-knot nematode( RKN) disease for tomato breeding through marker-assisted selection,a multiplex PCR reaction system was developed with tomato lines 1227( with ty-5 gene),VFNT( with Mi-1 gene) and segregation population F2 by analyzing the polymorphism of amplified fragment with redesigned molecular markers. The identification of the genotype and disease resistance in F2 population by multiplex PCR detection was 95% similar to that by field inoculation evaluation. The multiplex PCR system developed in this study is capable of simutaneous identification of ty-5 and Mi-1 in tomato.
出处
《江苏农业学报》
CSCD
北大核心
2016年第4期869-873,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省自然科学青年基金项目(BK20140739)
江苏省农业自主创新基金项目[CX(14)5012]
江苏省农业科学院基本科研业务专项[ZX(15)2003
ZX(15)4022]
国家自然科学青年基金项目(31401884)
关键词
番茄
番茄黄化曲叶病毒
根结线虫
抗性基因
多重PCR
tomato
tomato yellow leaf curl virus
root-knot nematode
resistance gene
multiplex PCR