戊糖片球菌对Caco-2细胞的黏附及黏附素的鉴定

The adhesion of Pediococcus pentosaceus to Caco-2 cell and identification of the adhesin

为了研究戊糖片球菌SR2-6对Caco-2细胞的黏附作用,采用不同的化学试剂和酶处理SR2-6,测定其表面成分黏附性质变化,并提取戊糖片球菌表面的细胞黏附素,进行成分和结构分析。同时测量了Caco-2细胞MUC3基因表达量的变化。结果显示,戊糖片球菌SR2-6能明显上调Caco-2细胞MUC3基因表达;戊糖片球菌SR2-6的肽聚糖参与了Caco-2细胞黏附的主要过程,该肽聚糖为α型肽聚糖,提取物主要由谷氨酸、丙氨酸、天冬氨酸、赖氨酸组成,总糖含量为(9.42±1.55)%,蛋白质含量为(4.61±1.88)%。戊糖片球菌SR2-6肽聚糖是一种抑制性黏附素,浓度越高,对SR2-6黏附Caco-2细胞的抑制越强,同时上调MUC3基因表达的作用也越强。

T o study the adhesion of Pediococcus pentosaceus S R 2 - 6 to Caco-2 cells,S R 2 - 6 was treated differently with chemical agents and enzymes. The adhesin involved in the adhesion was extracted and the components and structures were analyzed. Peptidoglycan of SR2-6 exerted a major role in the adhesion processes. The peptidoglycan,demonstrated as the a-type,was a kind of inhibitory adhesin,consisting of glutamic acid,malonic acid,aspartic acid and lysine,with total sugar content of （9.42±1. 55）% and protein content of （4.61±1. 88）%. The higher the concentration of peptidoglycan,the stronger the inhibition against the adhesions of SR2-6 to Salmonella and Caco-2 cells,and the stronger the up-regulation of the MUC3 gene..