丹参多酚酸盐对肺癌模型小鼠肺组织中VEGF和MMP-9表达的影响

Effects of Salvianolate on the Expression of VEGF and MMP-9 in Lung Tissue of Mice with Lung Cancer

目的:研究丹参多酚酸盐对肺癌模型小鼠肺组织中血管内皮生长因子(VEGF)和基质金属蛋白酶9(MMP-9)表达的影响。方法:取C57BL/6小鼠随机分为模型组(生理盐水)、阳性对照组(环磷酰胺50 mg/kg)和丹参多酚酸盐低、中、高剂量组(20、40、80 g/kg),每组10只。小鼠ip乌拉坦1 000 mg/kg,每周2次,连续4周建立肺癌模型;建模后各组小鼠ip相应药物,每日1次,连续给药14 d。比较各组小鼠行为、体征、体质量、抑瘤率、脾脏系数、胸腺系数及肺组织中血管内皮生长因子(VEGF)和基质金属蛋白酶9(MMP-9)表达。结果:模型组小鼠活动度消失,毛色暗淡无光泽,喜扎堆;阳性对照组和丹参多酚酸盐各剂量组小鼠上述现象均好转。阳性对照组和丹参多酚酸盐低、中、高剂量组小鼠的体质量分别为(21.01±2.95)g、(20.89±3.14)g、(21.03±3.02)g、(21.24±3.17)g,抑瘤率分别为(41.12±15.42)%、(36.92±10.42)%、(39.41±12.39)%、(37.19±10.39)%;与模型组比较,其余4组小鼠的脾脏系数、胸腺系数、VEGF(阳性对照组除外)和MMP-9表达均明显降低(P<0.05)。结论:丹参多酚酸盐对肺癌模型小鼠有明显的抑瘤作用,其作用机制可能与抑制VEGF和MMP-9表达相关。

OBJECTIVE： To study the effects of salvianolate on the expression of vascular endothelial growth factor （VEGF） and matrix metalloproteinase-9 （MMP-9） in lung tissue of mice with lung cancer. METHODS： C57BL/6 mice were randomly divid- ed into model group （normal saline）, positive control group （cyclophosphamide 50 mg/kg） and salvianolate low-dose, medi- um-dose and high-dose groups （20, 40, 80 g/kg） with 10 mice in each group. They were ig 1 000 mg/kg urethane, twice a day, for consecutive 4 weeks to establish lung cancer model. After modeling, they were given relevant medicine intragastrically once a day for consecutive 14 d. Mice behaviors, symptoms, body weight, tumor inhibition rate, spleen index, thymus index, and the ex- pressions of VEGF and MMP-9 in lung tissue among groups were compared. RESULTS： The activity of mice in model group disap- peared, dull coat color, preferred crowding together; the above-mentioned phenomenon improved in positive control group and sal- vianolate different dose groups. The body weight of mice in positive control group, salvianolate low-dose group, medium-dose group and high-dose group were （21.01 ± 2.95） g, （20.89 ± 3.14） g, （21.03 ± 3.02） g, （21.24 ±3.17） g; and the tumor inhibition rates were （41.12± 15.42）%, （36.92 ± 10.42）%, （39.41± 12.39）% and （37.19 ± 10.39）% ; compared with model group, spleen index, thymus index, and the expressions of VEGF （except for positive control group） and MMP-9 in other 4 groups decreased （P〈0.05）. CONCLUSIONS： Salvianolate shows obvious inhibitory effects on mice with lung cancer, which may be related to in- hibiting expressions of VEGF and MMP-9 in lung tissue.