喉癌组织核因子-κB诱导激酶表达临床意义研究

Expression and clinical significance of NF-κB inducing kinase in laryngeal cancer

目的核因子-κB诱导激酶(nuclear factor-κB inducing kinase,NIK)在多种肿瘤细胞中高表达,但其在喉癌中的表达情况目前尚不清楚。为此,本研究探讨NIK蛋白在喉癌组织中的表达及其临床意义。方法收集河北医科大学第三医院2000-01-01-2010-09-30接受手术治疗的63例喉癌患者的术中切除标本,所有患者均经术后病理确诊。采用免疫组织化学方法检测NIK蛋白在喉癌及癌旁正常组织中的表达水平,探讨NIK表达水平与患者的预后和临床病理特征的相关性。结果 NIK在喉癌组织中的阳性表达率为60.3%(38/63),显著高于癌旁正常组织的9.5%(6/63),χ2=35.76,P<0.001。NIK的表达与患者的性别(χ2=0.02,P=0.881)、年龄(χ2=0.027,P=0.867)、分化程度(χ2=0.14,P=0.932)和肿瘤部位(χ2=2.36,P=0.127)无关,而与患者的临床分期(χ2=13.7,P=0.003)和有无淋巴结转移(χ2=20.2,P<0.001)显著相关。NIK阳性表达的喉癌患者5年生存率为28.6%,显著低于阴性表达患者的80.9%,χ2=4.07,P=0.043。多因素Cox比例风险回归模型分析进一步显示,NIK蛋白表达水平为喉癌患者预后的独立影响因素,P=0.022。结论 NIK在喉癌组织中的表达明显升高,可能是影响患者预后的重要因素。

OBJECTIVE Nuclear factor-κB inducing kinase （NIK） is often highly expressed in tumor cells,but the clinical significance in laryngeal cancer has been unclear. The current study was to evaluate the expression of NIK in la- ryngeal cancer from protein level and the impact of NIK expression on clinicopathologic data. METHODS Specimens from 63 patients with laryngeal cancer managed in the Third Affiliated Hospital of Hebei Medical University between January 01,2000 and September 30,2010 were included in this study. Immunohistochemical staining was used to detect the expression of NIK in 63 cases of laryngeal cancer and 63 cases of normal esophageal mucosal tissues. All the specimens were confirmed by pathology for laryngeal cancer or esophageal normal larynx tissues. RESULTS The positive expres- sion rate of NIK in laryngeal cancer and normal larynx tissues were 60.3% （38/63） and 9.5% （6/63）, respectively. The positive expression rate of NIK in laryngeal cancer was significantly higher than those in normal larynx tissues （;（2 = 35.76,P〈0. 001）. The positive expression rate of NIK in laryngeal dancer was not related with the sex（X^2=0.02,P= 0. 881, age（X^2=0. 027, P= 0. 867）, tumor differentiation （X^2 = 0. 14, P= 0. 932） and tumor location（X^2 ： 2.36, P = 0.127）,but with clinical stage （X^2=13.7,P=0.003）and lymph node metastasis（x2=20. 2,P〈0. 001）. Patients with positive NIK expression had significantly lower five-year survival rate than those with tumors having positive NIK expres- sion（28.6%vs 80.9%, X^2 = 4.07, P= 0. 043）. Multivariate Cox regression analysis revealed that NIK overexpression was an independent prognostic factor for patients with laryngeal cancer （P=0. 022）. CONCLUSION The expression of NIK is increased in laryngeal cancer, which has certain reference value for estimating prognosis of laryngeal cancer patients.