酸性电解液对微弧氧化钛合金膜层及细胞附着的影响

Effects of Acidic MAO Electrolytes on Ti Alloy Coating and Osteoblast Adhesion

本文采用5%H2SO4和10%H3PO4电解液在钛合金表面通过微弧氧化方法制备多孔氧化膜。利用体外培养成骨细胞MG63的方法,评估了细胞在不同膜层上的早期附着能力。采用X射线衍射(XRD)、扫描电子显微镜(SEM)和能谱仪(EDS)研究了不同电解液中微弧氧化工艺的适用电压范围、膜层形貌、膜层的物相类型和膜层成分。研究结果表明:随着电压的增加,在两种电解液中的膜层孔洞尺寸逐渐变大,但孔洞数量减少;H2SO4中最佳氧化电压为120V,H3PO4中最佳电压为270V;H2SO4与H3PO4中制得的氧化膜在最佳电压下的平均孔隙直径分别为263.6nm和1.736μm。在H2SO4中的氧化膜层中出现锐钛矿和金红石相,H3PO4处理的氧化膜层中为无定型的氧化钛相。不同氧化膜内P与S的原子分数都随着电压升高而增大,最大原子分数分别为21.8%和4.13%。体外细胞培养过程中,在H2SO4电解液中不同电压下制得膜层上的细胞数量增长大于H3PO4,而在H3PO4中制得膜层上的细胞形态优于H2SO4。

Titanium oxide films were prepared with micro-arc oxidation method on Ti-6Al-4V in 5% H2SO4 and 10% H3PO4 electrolytes and MG63 cells were grown in co-culture on oxide films. The surface morphology and crystal structure of the oxide films under various voltages were first observed, and the influence of chemically modified surfaces of Ti-6Al-4V on the adhesion ability of MG63 cells was then analyzed. The phase component, morphologies and element content were characterized by means of XRD, SEM and EDS. The results showed that the best oxide voltages for H2SO4 and HaPO4 are 120V and 270V. The pore diameters under the best voltages in H2SO4 and H3PO4 are 263.6nm and 1. 736um, respectively. Oxide films consisting of rutile and/or anatase phases with porous structures are formed on Ti-6Al-4V after oxidizing in H2SO4, while amorphous titania with matrix phase is produced in H3PO4. The mole fractions of P and S keep rising with increasing voltage, and the largest mole fractions for P and S are 21.8% and 4.13%. Number of MG63 cells adhered to the surface of Ti-6Al-4V oxidized in H2 SO4 are higher than that in Ha PO4 as the voltage increased, but the cell shape exhibit more differentiated phenotype on the films oxidized in H3PO4.