陶瓷膜与醇沉等方法精制骨痹颗粒的药效学比较及其作用机理

Compare study on pharmacodynamic results and investigation of refining mechanisms with Gubi granule by using separation technologies of ceramic membrane,ethanol precipitation and other methods

通过比较3种方法精制中药复方骨痹颗粒的主要药效学研究结果,探讨分离技术对中药复方水提液精制的可行性及其分离机理.以中药复方骨痹颗粒为实验体系,分别以陶瓷膜分离、乙醇沉淀法及大孔吸附树脂法所得精制产物为考察样品,以小鼠足趾肿胀、耳廓肿胀及软骨细胞实验评价不同精制液在高、中、低剂量下的药效学指标.3种精制产物均对小鼠足趾肿胀和耳廓肿胀有不同程度的抑制效果,总体上,陶瓷膜组的抗炎效果在两种模型中的中、高低剂量下都呈现出较好的抗炎效果;3种精制产物对软骨细胞的促增值率存在显著性差异,其中陶瓷膜组优于醇沉组、树脂组.陶瓷膜组产物总体抗炎效果较好的机理之一可能是膜技术在达到精制效果的同时,可较完整保留中药复方药效物质的完整性,从而避免了原方中对软骨细胞具促增值作用的药效成分的损失.陶瓷膜分离技术作为中药复方水提液的精制工艺,在复方药效物质群集筛选方面具有其他分离技术不可比拟的优势.

To investigate three refinement methods and pharmacodynamic results on potency of Gubi granules and explore the feasibility and mechanism of separation technology to Chinese herbal compound purified water extract. Three different refinement methods e. d. ceramic membrane separation, ethanol precipitation and macroporous resin absorption were used. Three types of refined granule products were divided into high-, medium-, low-dose groups Mice toe-swollen assay, auricle-swollen assay, chondrocyte assay were implemented in order to compare the pharmacological activities of the 9 groups. Three types of refinement products all exerted inhibitory activities against toe-swollen and auricle-swollen. In general, ceramic membranes were thought to have a good anti-inflammatory effect of high-, medium-, low-dose groups in both models. A significant difference on the growth of value-added rate of chondrocytes was found from the three refined products in which the ceramic membranes was better ＂than ethanol precipitation and macroporous resin. The results showed that the membrane technology, achieved a good refining effect because it conserved the intact integrity of Chinese herbal formula, and was thought one of the reason for anti-inflammatory mechanism of ceramic membrane group product. Thus avoiding the loss of the medicinal ingredient of hondrocytes with promoting added value. The ceramic membrane separation technology as traditional Chinese medicine water extract solution purification process, in terms of comPound effective substance screening clusters had unparalleled advantage than other separation techniques.