不同方法提取荸荠皮多酚及清除DPPH自由基活性的比较研究

Different Methods for Extracting Water Chestnut Peel Polyphenols and Activity of Removal of DPPH Free Radical

通过不同方法提取荸荠皮多酚,测定其清除DPPH自由基活性的能力,得到半抑制浓度(IC50)来确定不同方法提取的荸荠皮多酚抗氧化活性的强弱。结果表明:几种不同方法提取的荸荠皮多酚均有较强的抗氧化能力,都比对照的几种合成抗氧化剂能力强;乙醇-硫酸铵双液相体系微波处理提取的荸荠皮多酚抗氧化活性相对更强,提取条件为:醇-水比为0.5,(NH_4)_2SO_4用量为0.30 g/m L,微波功率为288 W,提取时间为8 min,可获得稳定的双液相体系和较高的荸荠皮多酚含量。

Water chestnut peel polyphenols were extracted with different methods, Its ability to remove DPPH free radical was determinated. The water chestnut peel polyphenol antioxidant activity with different extracting methods were determinated by half inhibitory concentration （ IC50）. The results show that the water chestunt peel polyphenols from the several different methods have strong antioxidant capability, they are stronger than that of the control. The water chestnut peel polyphenol antioxidant activity is relatively stronger extracted with ethanol--ammonium sulphate aqueous two-phase system of microwave processing, the extracting conditions are ： ethanol-water ratio 0. 5 , （ NH4 ） 2SO4 dosage 0. 30g/mL, microwave power 288 W, extracting time 8 min, under these conditions, it can obtain stable aqueous two-phase system and high water chestnut peel polyphenol content.