Construction and Analysis of Asexual Mutant Population of Tetraploid Potato Longshu 7 Induced by EMS

EMS诱导四倍体马铃薯陇薯7号无性突变群体建立与分析（英文）

[Objective] This study was conducted to detect the variation induction ability of ethyl methanesulfonate （EMS）, to construct a mutant population. [Method] 0.6%, 1.2% and 2.4% EMS solutions were used for inducing 2 400 stem segments of test-tube plantlets of tetraploid potato Longshu 7, and an asexual mutant population including leaf, plant type, stem, stolon, glandular hair traits was constructed. [Result] Twenty two mutation types, i.e. etiolated plantlet, albino plantlet, leaf-color gradient, etiolated vein, wrapped bud, lobed leaf, crinkled leaf, deformed compound leaf, cluster, branch, top enlargement, top bifurcation, succulent, stolon distortion, stolon shift, early tuberization, hair stolon, stem enlargement, vine stem, foliage stem, glandular hair and whorled leaf types were observed in total. There were 52 mutant single plants in total, with a mutation rate of 21.67‰. [Conclusion] The glandular hair, clustered short-stem, branched stem and early tuberization types of mutants have higher application value, and are anticipated to be used in potato functional genome research, gene improvement and germplasm innovation.

[目的]检测甲基磺酸乙酯(EMS)的诱导变异能力,以建立突变体库。[方法]用0.6%、1.2%和2.4%的EMS诱导四倍体马铃薯陇薯7号试管苗茎段2 400个,建立了包含叶片、株型、茎秆、匍匐茎、腺毛等性状的无性突变群体。[结果]共发现了22种突变类型,分别为黄化苗、白化苗、叶色渐变、叶脉失绿、叶芽包裹、裂叶、卷缩叶、复叶畸形、簇生、顶端膨大、顶端分叉、肉质化、匍匐茎畸变、匍匐茎移位、早结薯、发丝匍匐茎、茎节膨大、多腺毛和轮生叶序。共52个突变单株,突变频率为21.67‰。[结论]腺毛、簇生矮秆、茎秆分支、早结薯等变异株系具有较高的应用价值,可望有效地被用于马铃薯功能基因组研究、遗传改良和种质创新。