Identification of Polysaccharides from Chinese Yam(Dioscoreae opposite “Qi”) by Infrared Spectroscopy

红外光谱法在蕲山药多糖分析中的应用（英文）

In this study, the structure of polysaccharides from Chinese yam （Dioscoreae opposite ＂Qi＂） was analyzed and identified by infrared spectroscopy within an infrared spectral range of 400-4 000 cm^-1, Characteristic absorption peaks were observed at 840 cm^-1 （α-glucosidic bond）, 759.21 cm^-1 （xylose）, 830.87 cm^-1 （rhamnose）, 812.81 cm^-1, 876.60 cm^-1 （mannose） and 842.64 cm^-1 （arabinose） in infrared spectra of polysaccharides from D. opposite ＂Qi＂, indicating that D. opposite ＂Qi＂ polysaccharides exist mainly in the form of α-glucosidic bonds, including xylose, rhamnose, mannose and arabinose. The experimental results could provide the basis for quantitative analysis, development and application of polysaccharides from D. opposite ＂Qi＂.

该研究采用波长为400~4 000 cm^(-1)的红外光谱对蕲山药多糖结构进行了分析鉴定,得到了波长为840 cm^(-1)代表α-糖苷键的特征吸收峰和波长为759.21、830.87、812.81与876.60 cm^(-1)等代表木糖、鼠李糖、甘露糖的特征吸收峰,以及波长为842.64 cm^(-1)代表阿拉伯糖的特征吸收峰,推测蕲山药多糖主要以α-糖苷键形式存在,主要包括木糖、鼠李糖、甘露糖、阿拉伯糖等。试验结果为下一步定量研究蕲山药多糖及其开发应用提供了基础数据。