褐藻多糖铁(Ⅲ)的制备及其性质测定

Studies on the Preparation,Properties of Alginate-Iron( Ⅲ) Complex

目的一种新型补铁剂——褐藻多糖铁(Ⅲ)的制备并测定其多项特性。方法通过单因素和响应面优化实验确定褐藻酸钠与氯化铁反应制备褐藻多糖铁(Ⅲ)的最佳工艺条件。用IR、DSC表征褐藻多糖铁(Ⅲ),其还原溶出性和释放率在模拟胃肠道中测定。结果褐藻酸钠与氯化铁质量比为1∶3.2,褐藻酸钠与枸橼酸钠质量比为1∶1.4,反应液p H为6.95,反应温度为80℃,反应3.29 h,制备得到的褐藻多糖铁(Ⅲ)中含铁量为19.57%;IR、DSC图谱表明褐藻酸钠中羟基与Fe3+发生了结合;褐藻多糖铁(Ⅲ)在生理p H范围内可溶解且易被维生素C还原,在模拟肠道中60 min的累积释放率大于80%。结论本实验所得褐藻多糖铁(Ⅲ)的制备工艺可行,产品含铁量高、溶解性好,具备作为新型补铁剂的基本属性。

OBJECTIVE To study preparation technology and properties of a new kind of iron-supplement of alginate-iron （ Ⅲ） complex. METHODS The preparation parameters of polysaccharide iron （Ⅲ） complex with sodium alginate and ferric trichloride were optimized by means of the single factor and response surface design. Alginate -iron （Ⅲ） complex was characterized by IR and DSC. Its reducibility and drug release percentage were determined in simulated gastrointestinal liquid. RESULTS Optimum condition follows ： alginate sodium to ferric trichloride with mass ratio of 1 ： 3.2 and alginate sodium to sodium citrate with mass ratio of 1 ： 1.4, which were reacted in pH 6. 95 of reaction liquid and at 80 ℃ of water bath lasting for 3.29 h and generated alginate-iron （ Ⅲ ） complex with the 19.57% of iron content. The IR and DSC indicated that the hydroxyl groups of alginate was combined with Fe（ Ⅲ ）. The alginate-iron（Ⅲ） complex could be dissolved and reduced easily by vitamin C in physiological pH conditions. And the cumulative release percentage was more than 80% within 60 min in stimulated gastrointestinal liquid. CONCLUSION The preparation technology of alginate-iron （ Ⅲ） complex was feasible. The product is high iron content and the solubility. And it can meet with the fundamental functions as a new kind of is supplement source.