氢化物发生-原子荧光光度法测定动物性食品中砷的含量

Determination of arsenic in animal foodstuff using hydride generation-atomic fluorescence spectrophotometry

为了检测动物性食品中重金属砷的含量，试验利用硝酸和高氯酸（4：1）对样品进行前处理，采用氢化物发生一原子荧光光度法对样品中的砷含量进行测定。结果表明：食品中的砷在0-10μg／L范围内具有良好的线性关系，得到线性方程Y=117．6773×C＋10．3255，相关系数（R2）=0．99978，检出限为0．033μg／L。在测定的样品中，天津市市售动物性食品鸡肉中的砷含量为（0．0584±0．0683）mg／kg，显著高于其他样品中的砷含量（P〈0．05），而猪肉、牛肉、猪肝脏、鸡蛋、鸭蛋中的砷含量差异不显著（P〉0．05），样品测定的相对标准偏差（RSD）为0．97％～2．15％，结果准确可靠。氢化物发生一原子荧光光度法灵敏度高、简单、快速、准确可靠，可以作为动物性食品中砷含量的检测方法。

To detect the content of heavy metal arsenic in animal foodstuffs, the samples were pretreated using nitric acid and perchloric acid （4：1）. And then the content of arsenic in the samples were determined using hydride generation -atomic fluorescence spectrophotometry. The results showed that the calibration curves of arsenic in animal foodstuffs had good linearity relationship in the range of 0 to10 μg/L , and a linear equation was obtained ： y = 117. 677 3 × C ＋ 10. 325 5 ; the correlation coefficient （ R2 ） was 0. 999 78 , and the limit of detection was 0. 033 μg/L. Among these samples , the content of arsenic in chicken from commercially available animal foodstuffs was （ 0. 058 4 ± 0. 068 3 ） mg/kg, which was significantly higher （P 〈 0.05 ） than that in the other samples. However, there were no significant differences （ P 〉 0.05 ） in the contents of arsenic among pork, beef, pork liver, eggs, and duck eggs. The relative standard deviation （RSD） of detected samples was 0.97% to 2.15%, and the results were accurate and reliable. The results indicate that the hydride generation - atomic fluores- cence spectrophotometry has the advantages of being simple, rapid, accurate, and reliable with high sensitivity, and can be used as a method for detecting the content of arsenic in animal foodstuffs.