缺失PUF家族基因的卵母细胞可以成熟和受精

Effects of removal of oocyte PUF family genes on female fertility and oocyte maturation

目的 :研究PUF家族基因[Pumilio1(Pum1)和Pumilio2(Pum2)]在雌性生育能力和生殖细胞减数分裂过程中的作用。方法:在Pum1基因位置携带lox P位点的Pum2基因全敲鼠的基础上,利用生殖细胞特异性表达的Cre-Vasa-Cre介导Pum1基因条件性敲除,以此获得Pum基因双敲小鼠模型Vasa-cre:Pum1F/FPum2-/-(VDKO)。同时,对Pum基因双敲小鼠进行生殖表型分析。结果:1交配实验结果表明,雌性VDKO小鼠的生育能力显著下降,但仍可以产生后代;2VDKO小鼠卵母细胞体外成熟过程不受影响。结论:Pum蛋白不影响小鼠卵子发生发育过程。

Objective：To investigate the role of PUF family genes [Pumiliol （Puml） and Pumilio2 （Pure2） ] in female fertility and meiotic maturation. Methods： We deleted the Pum genes by breeding mice harboring loxP sites in Puml （PumlF/F Pum2-/-） with DEAD box polypeptide 4 （Vasa） promoter-mediated Cre recombinase mice （VDKO）. Meanwhile. we analyzed the phenotype of VDKO mice. Results： Fertility test showed that female fertility of VDKO mice was reduced, but the mice could still produce offspring. However, in vitro meiotic maturation of VDKO oocytes was not affected. Conclusion： Pum proteins were not essential for mouse folliculogenesis or oocyte development.