拟南芥SNC1基因突变对植株细胞分生和抗氧化性状的影响

Effects of SNC1 Gene Mutation on Cell Proliferation and Antioxidation Character in Arabidopsis thaliana

以拟南芥snc1突变体、其抑制子突变体mos4snc1和Col-0野生型植株为材料,研究了SNC1基因突变对植株细胞分生和抗氧化性状的影响。结果表明,snc1植株平均比Col-0植株矮65.05%,其第3、4片真叶平均长度比Col-0的短53%,叶片平均宽度比Col-0的窄51%,角果荚平均短9.48%,平均种子数量少20.69%。与Col-0野生型植株相比,snc1植株第1、2片真叶细胞数目平均减少53.14%,而第3、4叶细胞数目平均减少61.88%;snc1突变体的SOD、POD、CAT酶活性和植株中花青素积累量均极显著高于Col-0野生型植株的,而snc1的抑制子突变体mos4snc1则恢复了野生型表型,其SOD、POD酶活性和花青素含量与Col-0野生型植株的没有显著差异。这说明SNC1基因突变严重影响了拟南芥植株的细胞分生和抗氧化性能力,导致细胞数目大幅度减少,株型矮小。

Wild type Col -Q,sncl mutant and its suppressor mos4sncl double mutant plants was used as materials to study the effects of SNC1 gene mutation on cell proliferation and antioxidation character in Arabidopsis thaliana. The results showed that the average plant height of sncl was 65. 05% shorter than that of Col - 0 wild type plants,with the average blade length and width of the third and 4th true leaf reduced by 53% and 51% ,average silique length and numbers of seeds per silique reduced by 9. 48% and 20. 69% Respectively. Compared with the wild type plants,cell number of the first and second true leaves decreased 53. 14% ,and that of the third and 4th leaves in sncl mutants decreased 61. 88%. The activity of superoxide dismutase （SOD） ,peroxidase （POD） and catalase （CAT） enzyme in sncl mutants was significantly higher than that of the wild type plants. The accumulation of anthocyanin in sncl was also significantly higher than that of the wild type plants. The mutated phenotype was restored to wild type in mos4sncl double mutant. As a result,there is no significant difference for the activity of SOD and POD between mos4sncl double mutant and wild type plants. It is suggested that the cell proliferation and antioxidation character is severely affected by SNC1 gene mutation which leads to the cell number reduction and plant drawf with sncl gene.