深圳2580例高危男性人乳头瘤状病毒基因型分析

Analysis of Human Papillomavirus Genotype in 2 580 Men Residing in Shenzhen

目的 探讨深圳门诊高危男性患者人乳头瘤状病毒（HPV）感染的分布状况及基因型特点,为男性HPV感染的干预提供理论依据。方法 采用多重PCR结合反向斑点膜杂交的基因芯片技术对2012年3月-2014年6月在北京大学深圳医院收集的2 580例门诊疑似HPV感染的高危男性患者的生殖道脱落细胞进行23种HPV基因分型检测。结果 2 580例样本中HPV阳性1 164例,阳性率为45.12%, 其中低危型、高危型及高低危混合型感染的阳性率分别为51.03%（594/1 164）,24.74%（288/1 164）,24.23%（282/1 164）。低危型以HPV6（46.29%）,HPV11（25.31%）,HPV43（18.77%）和HPV42（9.62%）为常见亚型; 高危型以HPV16（16.58%）,HPV52（12.59%）,HPV33（10.60%）,HPV58（7.98%）和HPV56（7.73%）为常见亚型。HPV感染以单一型感染为主（63.23%）,二重及多重感染分别为10.48%和26.29%。年龄分层结果显示,不同年龄组HPV阳性率差异无统计学意义（χ^2=6.98,P〉0.05）。结论 深圳市高危男性HPV感染率较高,HPV6,11,43,42为主要的低危亚型,HPV16,52,33,58,56为主要的高危亚型,感染以单一亚型为主。

Objective To assess the prevalence and genotype distribution of human papillomavirus （HPV） infection among men residing in Shenzhen,and provide the convincing evidence for the HPV intervention in men. Methods By using multiple PCR with reverse dot-blot gene chip technique,the 23 kinds of HPV gene types were detected in 2 580 male out-patients, who were suspected with HPV infection in Shenzhen Hospital of Beijing University from March 2012 to June 2014. Results Among 2 580 cases,1 164 male patents were HPV positive,and the overall HPV positive rate was 45.12%. The positive rates of low-risk,high-risk, both low and high risk HPV types were 51.03% （594/1 164）, 24.74% （288/1 164） and 24.23% （282/1 164）, respectively. The most commonly detected low-risk genotypes were HPV6 （46.29%）, HPV11 （25.31%） and HPV43 （18.77%）, respectively. The most frequently detected high-risk HPV genotypes were HPV16 （16.58%） ,HPV52 （12.59%）, HPV33 （10.60%）, HPV58 （7. 98%） and HPV56 （7.73%）, respectively. HPV infection was dominated by a single type infection （63.23%）, and the rates of double and multiple type infections were 10.48% and 26.29% respectively. Age stratification showed that HPV positive rate in different age groups had no statistically difference （Chi-square value 6.98,P〉0. 05）. Conclusion The HPV infection rate of high-risk men in Shenzhen was high. HPV6,11, 43,42 were the most commonly detected low-risk types; and HPV16,52,33,58,56 were the most commonly detected high- risk types. Most male patients were single type HPV infection.