大骨节病软骨中内质网应激性蛋白分子表达变化研究

Changed expression of protein molecule related to ER stress in KBD cartilage

目的:探讨活化转录因子6(ATF6)和内质网应激相关促凋亡蛋白C/EBP同源蛋白(CHOP)在大骨节病软骨组织中的表达变化及其与软骨损伤的关系。方法:收集7例大骨节病患者术后关节软骨标本(KBD组),7例骨关节炎病人关节软骨(OA组)作为疾病对照,6例正常软骨组织作为正常对照。采用SP免疫组化染色法检测三组关节软骨组织中AFT6和CHOP表达趋势,分析其关节软骨内阳性表达率差异。结果:KBD组和OA组中层ATF6阳性细胞表达率显著高于正常软骨组,而三组表层和深层软骨ATF6阳性细胞表达率无统计学差异。OA组软骨表层CHOP表达显著高于KBD软骨组和正常软骨组,而KBD组和正常组间比较无统计学差异;KBD组软骨和OA组中层CHOP阳性细胞表达率显著高于正常软骨组;OA组软骨深层阳性率高于KBD组。KBD组与OA组ATF6主要表达于中层,正常软骨组主要表达于中层和深层,KBD组与OA组CHOP主要表达于中层,正常软骨组CHOP在三层表达无统计学差异。结论:KBD软骨中层的ATF6和CHOP显著上调,表明CHOP高表达可能与大骨节病软骨损伤密切相关,而ATF6高表达与其软骨损伤后反应性细胞增殖有关。

Objective：To investigate the expression of ATF6 and CHOP in KBD cartilage changes and its relationship with KBD cartilage injury.Methods：7specimens of articular cartilage were collected from KBD patients under operation（KBD group）,7cases from patients suffered from osteoarthritis（OA group）as disease control,6normal cartilage as normal controls.Expression of AFT6 and CHOP as key regulators in ER stress of three groups articular cartilage was detected using immunohistochemical staining,significant differences expression among the articular cartilage was analyzed.Results：In middle layer,ATF6-positive expression rate in KBD group and OA group was significantly higher than this of normal cartilage group,while in the surface and deep,ATF6 positive cells among three groups was no statistical difference。In surface layer,CHOP-positive expression rate in OA cartilage was significantly higher than this of KBD and normal,while no statistical difference was found between KBD and the normal group;while in middle layer,CHOP-positive expression rate in KBD cartilage group and OA group was significantly higher than normal group,positive rate of deep in OA cartilage was higher than KBD group。ATF6of KBD and OA group was mainly expressed in the middle layer,whilemiddle and deep in normal cartilage,CHOP of KBD and OA group was mainly expressed in the middle layer.Conclusion：Both ATF6 and CHOP in middle layer of KBD cartilage was increased significantly,indicating that CHOP may be closely related to the KBD cartilage damage;while ATF6 was related with react Aive cell proliferation after cartilage injury.