青霉菌侵染前后哈密瓜转录组的构建及分析

Construction and Analysis of the Transcriptome Before and After Infected by Penicillium in Hami Melon Fruit

丰富的营养物质容易被青霉菌侵染,从而导致哈密瓜的腐坏变质。利用Illumina测序技术考察青霉病原菌侵染前后哈密瓜基因转录组水平的差异变化。利用Illumina平台测序并分析了哈密瓜的转录组信息。过滤后的Clean Reads包含4 947 555 420 nt的碱基数,对Clean Reads进行组装,获得了50 502条Unigene。在NR数据库中,以E value值10-5为截点进行BLAST比对,共有71.28%的Unigenes比对到该数据库中。但还有10 526条Unigene没有比对到NR、NT和Swiss-Prot数据库,可能是哈密瓜果实区别于其它物种而特有的基因,这需要进一步验证。将本次转录组测序得到的39 976个Unigene比对到KEGG数据库进行Pathway注释,共映射到不同的代谢通路128个。

Hami melon is famous with its unique flavor and rich nutrition. However,it is easily spoilt due to infected by Penicillium. The changes of transcriptome levels before and after infected by Penicillium in Hami melon were investigated by illumina paired-end sequencing. The results showed that the filtrated clean reads contained 4 947 555 420 nt nucleotides, further assembling the Clean Reads led to the product of 50 502 unigenes. A total of 71.28% unigenes were found in NR database using BLASTX comparison with an E-value cut-off of le＇5, whereas 10 526 unigenes were still found to no significant matches to any known protein in NR,NT and Swiss-Prot database. These may be the specific genes leading to the difference between Hami melon and other species and need to be further verified. The unigene metabolic pathway analysis was conducted on the 39 976 unigenes through comparing to KEGG database and 128 metabolic pathways were mapped. Transcriptional analysis will greatly improve the understanding of postharvest physiology characteristics of Hami melon at the molecular level. It will provide a practical and effective model for the future research,such as exploring the expression and regulation of important genes related to Penicillium pathogen resistance.