不同取浆时间的蜂王浆对小鼠免疫功能的影响

Effects of Royal Jellies with Different Harvesting Time on the Immune Functions of Mice

探讨了不同取浆时间所生产蜂王浆对小鼠免疫力的影响。将48 h和72 h取的蜂王浆(48 h RJ、72 h RJ)采用灌喂的方法给予Balb/c小鼠,每种蜂王浆分别设高剂量组9 000 mg/kg(48 h RJ-H、72 h RJ-H),中剂量组3 000 mg/kg(48 h RJ-M、72 h RJ-M),低剂量组1 000 mg/kg(48 h RJ-L、72 h RJ-L),同时设一个对照组。每次灌喂量为10 m L/kg,空白对照则灌以等量蒸馏水。迟发型变态反应实验经口连续灌胃30 d,灌喂结束后,用二硝基氟苯诱导实验小鼠迟发型变态反应,测定各组实验小鼠迟发型变态反应水平;碳廓清能力实验经口连续灌胃45 d,灌喂结束后,自小鼠眼内眦注入墨汁,观察各组实验小鼠碳廓清能力。各剂量组均未对小鼠脏器系数产生显著影响(P>0.05);48 h RJ-H组和72 h RJ 3个剂量组与对照组小鼠相比,耳片质量差差异显著(P<0.05);48 h RJ-H组、72 h RJ-M、H剂量组碳廓清指数K和吞噬指数a与对照组比,都存在差异显著(P<0.05)。表明48 h RJ和72 h RJ均具有增强小鼠免疫功能的作用,但72 h RJ优于48 h RJ。

This study aimed to explore effects of royal jellies having different harvesting time on the immunity of mice. Results showed that no significant effects （F〉0.05） on the ratios of organ/body weight of Balb/c mice were observed with three doses （9 000,3 000 and 1 000 mg/ kg） of 48 h or 72 h royal jellies. In the delayed type hypersensitivity experiment, feeding the highest dose of 48 h or three doses of 72 h royal jellies daily by oral gavage for 30 days to mice induced by dinitrofluorobenzene resulted in remarkable effects （P 〈0.05） on the weight differences of ear slices. For the carbon clearance ability experiment,the clearance index and phagocytic index of mice fed with the highest dose of 48 h,the medium or highest doses of 72 h royal jellies daily by oral gavage for 45 days and then injected with ink from the eye canthi were statistically significant （P〈0.05） compared to those of the control group. Hierefore, both 48 li and 72 h royal jellies could enhance the immunity of mice witii tiie laer being superior to tiie former.