无锡城乡年轻居民肠道菌群多样性研究

Study on the Diversity of Intestinal Flora of Young Rural and Urban Residents in Wuxi

采用PCR和DGGE相结合的技术,研究了生活于无锡城市和乡村的青年志愿者肠道菌群的多样性。基于DGGE指纹图谱,使用聚类和PCA分析对志愿者肠道微生物相似性进行分析,使用Shannon-Weine多样性指数(H)、丰度(S)和均匀度(EH)对志愿者肠道微生物多样性进行分析,对图谱中具有代表性的共性和特异性条带进行胶回收和测序以分析志愿者肠道微生物组成;基于PCR技术在种水平上对城乡志愿者肠道内乳杆菌属(Lactobacillus)和双歧杆菌属(Bifidobacterium)多样性进行定性分析。结果显示,无锡城乡青年居民间肠道微生物群落结构存在分开趋势,相似性小于城市或乡村居民内部;多样性方面差异不显著;不动杆菌属、双歧杆菌属、葡萄球菌属、乳杆菌属、梭菌属和瘤胃球菌属为城乡青年居民肠道内的共有菌属;乳杆菌属Lactobacillus(L.)和双歧杆菌属Bifidobacterium(B.)各类菌群在城乡居民肠道内分布差异不显著,其中L.plantarum,L.casei,L.salivarius和L.acidophilus以及B.longum,B.breve,B.animalis和B.adolescentis分别为无锡青年居民肠道内的优势乳杆菌和双歧杆菌。综上分析,初步揭示了无锡城市和乡村青年居民肠道微生物多样性差异不显著。

The gut microbial diversity of young people living in rural and urban Wuxi was studied by the combination of polymerase chain reaction （PCR） and denaturing gradient gel electrophoresis （DGGE）. Based on the DGGE pro file, the similarity of volunteers was performed by analysis of clustering and PCA. On the other hand, the diversity was evaluated by the Shannon-Weine index, richness and eveness. The composition of gut microbiota was further characterized by sequencing of the common and special bands of the lanes in DGGE profile. Based on the PCR, the diversity of Lactobacillus and Bifidobacterium species in the gut of rural and urban volunteers was analyzed. The results showed that the structure of gut microbiota between rural and urban residents of Wuxi appeared a separated tendency, and the similarity of intestinal flora between them was lower than that of urban or rural residents alone. Furthermore,the diversity between them were not significantly different. The genus of Acinetobacter, Bifidobcicterium, Staphylococcus ,LactobacUlus , Clostridium and Ruminococcus were the common bacteria in the gut of young urban and rural residents. All kinds of bacteria of Lactobacillus （L.） and Bifidob acterium（B.） in the gut were not significantly different in rural and urban residents. And the species of L. plantarum, L. casei, L. salivarius , L. acidophilus , B. longum, B. breve , B. animalis and B. adolescentis were the predominant Lactobacillus and Bifidobacterium bacteria, respectively. On the base of the above comprehensive analysis,it can be preliminarily draw a conclusion that the diversity of gut microbiota was not significantly different between the young residents from rural and urban Wuxi.