子痫前期患者oxLDL、8-isoprostane、MDA和MIF的检测及意义

Detection and Significance of OxLDL,8-Isoprostane,MDA and MIF in Patients with Preeclampsia

目的探讨子痫前期患者氧化低密度脂蛋白(oxidatively modified low density lipoprotein,oxLDL)、8-异前列腺素(8-isoprostane)、丙二醛(malondialdehyde,MDA)和巨噬细胞移动抑制因子(macrophage migration inhibition factor,MIF)的检测及其临床意义。方法选择子痫前期患者49例,其中轻度子痫前期患者(轻度子痫前期组)26例,重度子痫前期患者(重度子痫前期组)者23例。选择接受剖宫产术的正常妊娠晚期孕妇(对照组)30例。检测3组胎盘组织中MIF mRNA的表达水平及血浆oxLDL、8-isoprostane、MDA的水平,并对轻度、重度子痫前期2组血浆oxLDL水平与胎盘组织中MIF mRNA表达水平的相关性进行分析。结果与对照组比较,轻度、重度子痫前期2组血浆oxLDL、8-isoprostane、MDA水平及胎盘组织中MIF mRNA表达水平均明显升高(均P<0.01);与轻度子痫前期组比较,重度子痫前期组血浆oxLDL、8-isoprostane、MDA水平及胎盘组织中MIF mRNA表达水平均显著升高(均P<0.01)。轻度、重度子痫前期2组血浆oxLDL水平与胎盘组织中MIF mRNA表达水平均呈正相关(r=0.85、0.87,均P<0.05)。结论子痫前期患者胎盘组织中MIF及血浆oxLDL、8-isoprostane、MDA的过度表达,可能参与子痫前期的发生发展。

Objective To investigate the detection and clinical significance of oxidatively modi-fied low density lipoprotein（oxLDL）,8-isoprostane,malondialdehyde（MDA）and macrophage mi-gration inhibition factor（MIF）in patients with preeclampsia.Methods The expression of MIF mRNA in placenta and the levels of oxLDL,8-isoprostane and MDA in plasma were measured in 26 patients with mild preeclampsia （MPE group）,23 patients with severe preeclampsia （SPE group）and 30 normal pregnant women（control group）.The relationship between oxLDL levels in plasma and MIF mRNA expression was analyzed in MPE and SPE groups.Results Plasma levels of oxLDL,8-isoprostane and MDA and placenta expression of MIF mRNA in MPE and SPE groups were higher than those in control group,and those in SPE group were higher than those in MPE group（P 〈0.01）.The levels of oxLDL in plasma were positively correlated with the expression of MIF mRNA in placenta in both MPE and SPE groups（r=0.85 and 0.87,respectively;P 〈0.05）.Conclusion Overexpression of placenta MIF and plasma oxLDL,8-isoprostane and MDA may be involved in the pathogenesis of preeclampsia.