多发性骨髓瘤患者Survivin、VEGF表达及其相关性

The expression of Survivin and VEGF in multiple myeloma and their relationship

目的:研究多发性骨髓瘤(MM)患者血清及骨髓Survivin、VEGF表达及意义。方法:酶联免疫法(ELASA)检测60例初诊多发性骨髓瘤患者及30例对照组患者血清Survivin、VEGF表达情况;免疫细胞化学法(SABC法)检测骨髓单个核细胞中Survivin、VEGF阳性率。酶联免疫法(ELASA)检测VAD联合沙利度胺治疗4周期后患者血清Survivin、VEGF水平,与治疗前比较。结果:初诊多发性骨髓瘤患者血清Survivin、VEGF表达水平较对照组明显升高;初诊骨髓瘤患者骨髓单个核细胞Survivin、VEGF阳性率较对照组明显提高;经统计学分析,P<0.05,差异有统计学意义。治疗后患者,血清Survivin、VEGF在不同疗效组有差异,疗效良好组与疗效较差组差异比较,P<0.05。骨髓中Survivin与VEGF表达阳性率呈正相关,与疾病疗效呈负相关。结论:Survivin及VEGF在多发性骨髓瘤患者中表达增加,二者表达水平与疾病疗效密切相关。二者存在协同关系,可作为评估疗效、预后指标。

Objective： To study the expression of Survivin and VEGF in mult iple myeloma and their relationship. Methods： To select sixty cases untreated patients with mult iple myeloma and thri ty cases person as control group, ELISA detect serum Survivin and VEGF protein levels,SABC detect the postive ratio of Survivin and VEGF in bone marrow mononuclear cells. ELISA detect serum Survivin and VEGF protein levels afte four chemotherapy with VADT program. Results ： The expression level of newly diagnosed mult iple myeloma patient serum Survivin and VEGF was significantly higher than the control group, the difference was statistically significant. In bone marrow mononuclear cells of newly diagnosed myeloma patients, Survivin and VEGF positive ratio were significantly higher than the control group,P 〈0.05. After four chemotherapy with VADT program,patient serum Survivin and VEGF protein levels were significantly lower than before chemotherapy, P 〈0. 05. Survivin and VEGF expression was positively correlated in-multiple myeloma patient bone marrow, negatively correlated with disease efficacy. Conclusion ： The expression of Sur-vivin and VEGF was increased in patients with multiple myeloma, closely related to the efficacy. They were corporated with each other, could be used to assess effect and prognosis.