反相高效液相法测定人血浆中头孢美唑浓度

Determination of the Concentration of Cefmetazole in Human Plasma by Reversed Phase High Performance Liquid Chromatography

目的：建立反相高效液相-紫外检测法测定人血浆中的头孢美唑浓度。方法内标法定量,以头孢唑啉为内标,采用YMC-C18（150mm ×4．6mm,5μm）柱,以0．1mol/L NaAc（冰醋酸调 pH =6．88）：乙腈=85：15为流动相,流速0．9mL/min,柱温为室温,紫外检测波长为272nm ,直接沉淀蛋白后反洗进样。结果标准曲线线性范围为：0．25~100μg/mL,线性=0．99995；最低定量限为0．25μg/mL；预处理回收率为：76．48%~99．65%；方法回收率为99．84~104．73%；日内RSD在0．68%~1．83%之间,日间RSD在2．06%~6．40%之间。结论该方法操作简单,结果准确,重现性好,可用于人血浆中的头孢美唑的含量测定和药动学研究。

Objective To establish a RP-HPLC method for the determination of the concentration of the Cefmetazole in human plasma. Methods Internal standard quantitative method,with cefazolin as internal standard,the YMC-C18（150mm × 4. 6 mm,5μm）column,with 0. 1mol/L NaAc（acetic acid adjusting pH=6. 88）：acetonitrile ratio of 85：15 as mobile phase,flow rate 0. 9ml/min and column temperature at room temperature,the UV detection wavelength for 272 nm,direct precipitation protein after backwashing inlet. Results The linear range of the standard curve is：0. 25 to 100μg /mL,linear=0. 99995;quantitation limit was 0. 25g/mL;pretreatment recovery for 76. 48% ~99. 65%;recovery rate was 99. 84 to 104. 73%;intra day RSD between 0. 68% ~1. 83% between day RSD between 2. 06% ~6. 40%. Conclusion The method is simple,accurate and reproducible,and can be used for the determination of the content of the content of the plasma and the pharmacokinetics of the drug in human plasma.