银翘袋泡茶的质量控制方法研究

Quality Standard for Yinqiao Teabags

目的建立银翘袋泡茶的质量控制方法。方法采用显微鉴别法鉴别方中的金银花;采用薄层色谱法对方中的金银花、广藿香进行定性鉴别;采用高效液相色谱法测定方中连翘苷的含量。结果金银花的花粉粒显微特征清晰,专属性好;金银花、广藿香的薄层色谱斑点清晰、分离度好,且各阴性对照无干扰;连翘苷在进样量为0.51~3.06μg范围内线性关系良好（r=0.999 9）,平均加样回收率为98.85%,RSD为1.51%（n=6）。结论该方法专属性强、灵敏度高、重复性好,可用于银翘袋泡茶的质量控制。

OBJECTIVE To establish the quality standard for Yinqiao teabag. METHODS Lonicerae japonicae flos was identified under microscope.Thin layer chromatography （TLC） was used to identify Lonicerae japonicae flos and Pogostemonis herba in Yinqiao teabags. The content of forsythin was determined by high performance liquid chromatography （ HPLC ）. RESULTS he resin passage of Lonicerae japonieae flos was clear and typical. The TLC spots were clear with good resolution and free of interference of negative samples.The forsythin showed good linear relationship in the range of 0.51-3.06 μg （r = 0.999 9）. The average recovery was 98.85%, RSD= 1.51% （n=6）. CONCLUSION The method is simple,specific and durable.lt can be used for the quality control of Yinqiao teabags.