竹节蓼石油醚部位抗肿瘤活性组分研究及其成分GC-MS分析

Study of Anti-Tumor Activity Fraction of Petroleum Ether Extracts from Homalocladium Platycladum and GC-MS Analysis of Its Components

目的研究竹节蓼石油醚部位筛选出的活性组分C_(6-8)、C_(25-28)体外抗肿瘤作用及其化学成分。方法采用MTT法研究其对肿瘤细胞PC-3和A549的体外增殖抑制活性,通过气相-质谱联用法(GC-MS)结合NIST 11标准质谱图库检索分析其化学成分,应用色谱峰面积归一化法计算各成分的相对百分含量。结果组分C_(6-8)对PC-3,C_(25-28)对PC-3、A549表现了较好的细胞增殖抑制活性;从组分C_(6-8)分离得到10个成分,鉴定出7个化合物,其中主要成分为羽扇豆醇(90.77%);从组分25-28分离鉴定出10个化合物,主要成分为γ-谷甾醇(56.11%)、L(+)-抗坏血酸-2,6-二棕榈酸酯(25.26%)、豆甾醇(9.89%)。结论组分C_(6-8)、C_(25-28)具有体外抗肿瘤活性,其主要成分为羽扇豆醇、γ-谷甾醇、豆甾醇。

OBJECTIVE To study the in vitro anti-tumor effect and its components of C6-8, C25-28 fiactions screened from petroleum ether extracts of homalocladium platycladum. METHODS The anti-treMor effect of the fractions on tumor cell line PC-3 and A549 in vitro was evaluated by MTT assay, and its components was identified by gas chromatography-mass spectrometry （GC-MS） combined with NIST I 1 standard MS library,relative percentage content of every compound was calculated by chromatographic peak area normalization method. RESULTS The C25-28 was significantly inhibited the growth of PC-3 and A549 with dose-dependent manner, C6.s only had inhibitory effect on the proliferation of PC-3. There were 10 components separated from C6_8 and 7 of them were identified, the major component was lupeol （90.77%）. There were 10 components separated and identified from C6-8,and the major components ware γ- sitosterol （56.11%）, L-（ ＋）-Ascorbic acid 2, 6-dihexadecanoate （ 25.26%）, stigmasterol （9.89%）. CONCLUSION C6-8, C25-28 fractions has anti-tumor activity in vitro, and the major components of them were lupeol, γ-sitosterol, stigmasterol.