梅归参颗粒的质量标准研究

A study on quality standard of Meiguishen granules

目的：建立梅归参颗粒的定性鉴别和含量测定方法。方法：采用薄层色谱（TLC）法对方中所含黄连、辛夷、白芷进行鉴别;采用高效液相色谱（HPLC）法测定方中枸橼酸的含量。结果：建立的薄层色谱法可较好地鉴别出黄连、辛夷、白芷,阴性无干扰;枸橼酸在1.025-10.248μg范围内呈良好的线性关系,r=0.9996,平均回收率为97.23%,RSD=1.44%。结论：所建立的TLC和HPLC方法准确、具有专属性、能够重现,并且操作简便,可将其用于梅归参颗粒的质量控制。

Objective： To investigate the methods for qualitative identification and content determination of Meiguishen granules. Methods： Thin- layer chromatography（ TLC） was used for the identification of Coptis chinensis,Flos Magnoliae,and Radix Angelicae Dahuricae in the prescription,and high- performance liquid chromatography（ HPLC） was used for the determination of citric acid. Results： The established TLC method identified Coptis chinensis,Flos Magnoliae,and Radix Angelicae Dahuricae well without negative interference. Citric acid showed a good linear relationship within the range of 1. 025- 10. 248 μg（ r = 0. 9996）,and the average recovery rate was97. 23%（ RSD = 1. 44%）. Conclusion： The established TLC and HPLC methods are accurate,specific,simple,and convenient,and therefore,they can be used for quality control of Meiguishen granules.