苗药野草香中黄酮类成分抗氧化活性研究

Antioxidant Activities of Flavonoids from Miao drug Elsholtzia cypriani(pam p.)C.Y.Wu et S.Chow

目的:对苗药野草香中提取分离的黄酮类成分进行抗氧化活性研究。方法:采用DPPH和ABTS两种方法测定。结果:木犀草素(2)、木犀草素-7-O-β-D-吡喃葡萄糖苷(3)、槲皮素(5)、槲皮素-3-O-β-D-吡喃半乳糖苷(6)、槲皮素-3-O-α-L-鼠李糖苷(7)对DPPH自由基和ABTS自由基均有一定的清除活性(IC_(50)分别为9.25±0.48、11.42±0.54、11.23±0.18、14.87±1.07、39.48±1.16和4.69±0.21、4.13±0.03、3.44±0.09、8.52±0.27、23.58±0.15)。结论:综合两种测定方法结果,化合物(2)和(5)的抗氧化能力最好。

Objective:To study the antioxidant activities of flavonoids from Miao drug Elsholtzia cypriani(pam p.)C.Y.Wu et S.Chow.Methods:The antioxidant and radical scaveging activity of all compounds were evaluated by DPPH and radicals cavenging assay.Results:luteolin(2),luteolin-7-O-β-D-gluco-pyranoside(3),quercetin(5),quercetin-3-O-β-D-galactopyranoside(6),quercetin-3-O-α-L-rhamnoside(7)possess the ability of clearance to both DPPH free radicals and ABTS free radicals,inhibition rate are 9.25±0.48、11.42±0.54、11.23±0.18、14.87±1.07、39.48±1.16和and 4.69±0.21、4.13±0.03、3.44±0.09、8.52±0.27、23.58±0.15.Conclusion:radical scavenging assay displayed that compounds(2)and(5)owned the most strong activity.