超声空化法制备脱细胞血管支架生物学相容性的实验研究

Experimental research of biocompatibility of the decellularized vascular scaffolds treated by ultrasound cavitation

目的检测经强度为300 W超声处理后的脱细胞血管支架的生物相容性。方法无菌条件下,采取新鲜兔股动脉反复冻溶+超高压处理后,利用核酸酶进行消化,脱去残留细胞碎片形成脱细胞血管支架。将强度为300 W的超声作用于脱细胞血管支架,并对处理后支架材料的生物学相容性进行检测和观察。结果经强度为300 W超声处理后的支架材料,其疏松化程度在明显提高的同时未发现明显的细胞毒性,且种子细胞可以长人材料内部。结论超声空化法处理后的脱细胞血管支架材料具有较好的组织相容性,可以为组织工程血管支架的研究提供新的思路和方法。

Objective To detect the biocompatibility of decellularized vascular scaffolds after 300 watts sonication. Methods Under sterile conditions, fresh blood from the femoral artery was isolated from rabbits and then nuclease digestion was performed after treatment with repeated freezing-thawing cycles and superten- sion. The decellularized vascular scaffolds were formed after removal of rudimental cell debris. Ultrasonic waves at an intensity of 300 watts were used to treat the scaffolds and then the biocompatibility of the treated scaffolds was detected and observed. Results With the 300 watts intensity ultrasonic treatment, a looser structure of the scaffolds was found without apparent cytotoxcity and seed cells could grow easily inside materials. Conclusi on The decellularized vascular scaffolds treated by the ultrasound cavitation method may provide a new method to obtain the decellularized vascular scaffolds with better biocompatibility.