神经节苷脂和神经生长因子在大鼠不同严重程度脑损伤治疗中的协同作用

Synergy between GM1 and NGF on the severity of brain injury in rats

目的观察神经节苷脂(GM1)和神经生长因子(NGF)对患有不同严重程度脑损伤大鼠的治疗作用,以探讨GM1与NGF可能存在的协同作用以及两者治疗效果的差异性。方法 140只10周健康的雄性SD大鼠分为正常组、假手术组、模型A组、模型B组、模型C组,使用电子颅脑损伤仪(e CCI)制备模型A/B/C,打击深度均为3 mm,打击速度分别为3、4、5 m/s。其中模型A/B/C组再分以空白组、GM1治疗组、NGF治疗组和GM1+NGF治疗组。给药后分别在24、48、72 h用ELISA方法检测血清中S100B和神经元特异性烯醇化酶(NSE)的含量。结果损伤发生后第24小时各组S100B和NSE含量均达到最高点,随后呈下降趋势;同一模型不同治疗组内,GM1+NGF治疗组下降趋势最显著(P<0.05);不同模型间,中度脑损伤模型的GM1+NGF治疗组下降最为显著(P<0.05)。结论GM1和NGF联合使用治疗比单独使用GM1或者NGF效果要好,两者存在协同作用;而且GM1和NGF联合使用治疗对于中度脑损伤情况效果最佳。

Objective To observe the therapeutic effect of ganglioside （GM1） and nerve growth factor （NGF） on the severity of brain injury in rats, and to explore the synergy and the difference of therapeutic effect between GM1 and NGF. Methods A total of 140 10 weeks healthy male rats were randomly divided into 5 groups： normal group, sham group, model A group, model B group and model C group, then the traumatic brain inJury model were prepared by electric Cortical Contusion Impactor （eCCI）, the hit depth was 3 ram. the hit speed of model A/B/C was 3 m/s, 4 m/s and 5 m/s respectively. Then the model A/B/C was divided into 4 groups： blank group, GM1 group, NGF group and GMI＋NGF group respectively. ELISA method was used to detect serum S100B and NSE concentration after treatment at 24 h, 48 h and 72 h respectively. Results S100B and NSE levels in each group had reached the highest point at 24 h after injury, and then decreased. Different treatment groups within the same model, GM1 ＋NGF treatment group decreased the most significantly （P 〈 0.05））; between different models, GMI＋NG treatment group in moderate brain injury model decreased the most significantly （P 〈 0.05）. Conclusion The therapeutic effect of GMI＋NGF treatment group is better than GM1 or NGF, two drugs have synergistic brain protection, and the best therapeutic effect of GMI＋NGF is to moderate brain injury model.