艾叶挥发油·总黄酮和鞣酸加压协同萃取研究

Pressurized Synchronously Extraction of Volatile Oil,Total Flavonoids and Tannic Acid from Artemisia argyi

[目的]考察加压协同萃取艾叶中挥发油、总黄酮和鞣酸的最佳工艺条件。[方法]采用加压辅助溶剂萃取,从艾叶中同步提取挥发油、总黄酮和鞣酸,通过单因素试验和正交试验优化工艺条件。[结果]加压对艾叶化学成分的提取率影响显著;确定加压溶剂萃取艾叶中挥发油、总黄酮和鞣酸的最佳工艺条件为溶剂浓度70%、固液比1∶30(g/m L)、萃取压力0.9 MPa、萃取时间40 min、萃取温度90℃,在此试验条件下,挥发油、总黄酮和鞣酸的提取率分别为0.852%、4.660%、6.790%。[结论]与提取艾叶化学成分的其他方法相比,加压溶剂萃取可实现艾叶中挥发油、总黄酮和鞣酸的同步提取。该工艺条件稳定,提取时间短,溶剂利用率高,艾叶中3种成分的提取效果较理想。

[Objective] To investigate the optimal technological condition for pressurized synchronously extraction of volatile oil,total flavonoids and tannic acid from Artemisia argyi. [Method] By using pressurized extraction,the volatile oil,total flavonoids and tannic acid were extracted synchronously from A. argyi. The extraction technology was optimized by single factor test and orthogonal test. [Result] Pressurization had significant effects on the extraction rate of chemical composition in A. argyi. The optimal extraction technology for volatile oil,total flavonoids and tannic acid from Artemisia argyi was as follows： 70% ethanol concentration,1∶ 30（ g / m L） solid-liquid ratio,40 min extraction time,90 ℃ extraction temperature,and 1. 0 MPa extraction pressure. Under these conditions,the extraction rate of volatile oil,total flavonoids and tannic acid were 0. 852%,4. 66% and 6. 79%,respectively. [Conclusion]Compared with other extraction methods of chemical components from A. argyi,pressurized extraction can realize simultaneously extraction of volatile oil,total flavonoids and tannic acid from A. argyi. This technological condition is stable,the extraction time is short and solvent utilization is high. Extraction effects of the three components in A. argyi leaves were relatively ideal.