洋葱伯克霍尔德菌携带整合子的分布与耐药性关系

Identification and molecular epidermiology of Burkholderia cepacia

目的研究临床分离洋葱伯克霍尔德菌中Ⅰ类整合子的流行现状以及其耐药性,为防控耐药菌形成和扩散提供理论依据。方法采用基因扩增技术和药敏试验方法,对某医院临床分离洋葱伯克霍尔德菌复合群进行检测与分析。结果从确认的54株洋葱伯克霍尔德菌复合群中,检出l6株携带整合酶Ⅰ,阳性率为29.63%;检出10株携带整合子Ⅰ,阳性率为18.52%;未检出Ⅱ类整合子和整合酶基因阳性菌株。Ⅰ类整合酶阳性菌株对左氧氟沙星和头孢他啶等抗菌药物的耐药率显著高于整合酶阴性菌株。结论洋葱伯克霍尔德菌携带Ⅰ类整合子与多重耐药具有相关性。

Objective To study the epidemic status and drug resistance of Burkholderia cepacia in order to provide refer- ence for the spread of drug resistant bacteria. Methods Gene amplification technology and drug sensitivity test were used to detect and analyze the compound group of Burkholderia cepacia in a hospital. Results 16 strains carrying the class I in- tegrase genes were detected from 54 strains of Burkholderia cepacia,and the positive rate was 29.63% ;10 strains carrying class I integrons were detected and the positive rate was 18.52% ;while positive strains carrying class II integrons and the II integrase gene weren＇ t detected. The resistant rates of the positive strains of class I integrase geue positive strains were sig- nificantly higher than those of the integrase gene negative strains. Conclusion Class I integron is associated with multiple drug resistance of Burkholderia cepacia.