摘要
Owing to the recent advances in sequencing technology, genetic mapping of mutations has been revolutionized by whole-genome deep sequencing and bulk segregant analysis （Schneeberger et al., 2009; Abe et al., 2012）. Once the mapping population for a mutant is available, genetic analysis can be simplified by using the pool of F2 plants with mutant phenotype and the pool of those showing wild-type phenotype. Since most mutant phe- notypes are of qualitative character and follow simple inheritance patterns, genome-wide comparisons between the two DNA pools could localize the causal gene within a small genomic region. In most cases, the causative mutation （e.g., an in-frame indel） can be detected as well because there are only a limited number of sequence differences between the mutant and its wild-type. Hence, rapid mutant mapping via genomics ap- proaches is now becoming practical and cost-effective.
Owing to the recent advances in sequencing technology, genetic mapping of mutations has been revolutionized by whole-genome deep sequencing and bulk segregant analysis （Schneeberger et al., 2009; Abe et al., 2012）. Once the mapping population for a mutant is available, genetic analysis can be simplified by using the pool of F2 plants with mutant phenotype and the pool of those showing wild-type phenotype. Since most mutant phe- notypes are of qualitative character and follow simple inheritance patterns, genome-wide comparisons between the two DNA pools could localize the causal gene within a small genomic region. In most cases, the causative mutation （e.g., an in-frame indel） can be detected as well because there are only a limited number of sequence differences between the mutant and its wild-type. Hence, rapid mutant mapping via genomics ap- proaches is now becoming practical and cost-effective.
