摘要
The single-molecule real-time （SMRT） sequencing platform pre- sented by Pacific Biosciences （PacBio） is regarded as a third- generation sequencing technology （Eid et al, 2009; Roberts et al., 2013）. PacBio delivers long reads from several to tens of kilobases （kbs）, which are ideal for filling unsequenced gaps due to unusual sequence contexts, such as high^GC content or repeat-rich regions （Bashir et al., 2012; Berlin et al., 2015; Chaisson et al., 2015）. PacBio long reads are also favorable for detecting large DNA fragments harboring structural variations （SVs）, such as inversions, translocations, duplications, and large insertions/deletions （indels） （Ritz et al., 2010; English et al., 2014）.
The single-molecule real-time （SMRT） sequencing platform pre- sented by Pacific Biosciences （PacBio） is regarded as a third- generation sequencing technology （Eid et al, 2009; Roberts et al., 2013）. PacBio delivers long reads from several to tens of kilobases （kbs）, which are ideal for filling unsequenced gaps due to unusual sequence contexts, such as high^GC content or repeat-rich regions （Bashir et al., 2012; Berlin et al., 2015; Chaisson et al., 2015）. PacBio long reads are also favorable for detecting large DNA fragments harboring structural variations （SVs）, such as inversions, translocations, duplications, and large insertions/deletions （indels） （Ritz et al., 2010; English et al., 2014）.
