正交试验法优选复方白芍肠康颗粒提取工艺

Optimization of Extraction Technology for Fufang Baishaochangkang Granules by Orthogonal Design

目的:优选复方白芍肠康颗粒提取工艺。方法:以芍药苷含量及干膏率为评价指标,通过正交试验考察加水量、煎煮时间、煎煮次数对水提工艺的影响;以厚朴酚含量及干膏率为评价指标,通过正交试验考察乙醇提取分数、乙醇用量、提取时间和提取次数对醇提工艺的影响。采用HPLC测定芍药苷、厚朴酚的含量。结果:最佳水提工艺为10倍量水,提取2次,每次1.5 h;芍药苷提取量为24.28 mg·g^(-1),干膏率为34.05%;最佳醇提工艺为8倍量60%乙醇,提取2次,每次1.5 h;厚朴酚提取量为13.07 mg·g^(-1),干膏率为19.71%。结论:优选的提取工艺稳定可行,为复方白芍肠康颗粒的合理开发提供参考。

Objective： To optimize extraction technology of Fufang Baishaochangkang Granules. Methods： Taking content of paeoniflorin and dry extract rate as evaluation index, orthogonal test was employed to investigate effects of the amount of water, extraction time and times on water extraction process. With contents of magnolol and dry extract rate as evaluation index, orthogonal test was adopted to optimize ethanol extraction process with ethanol concentration, ethanol amount, extraction time and times. Content of paeoniflorin and magnolol were determined by HPLC. Results： Optimum water extraction conditions were as follows ： decocted twice with 10 times the volume of water 1.5 h per time. Extracting amount of paeoniflorin was 24. 28 mg.g^-1, dry extract rate was 34.05%. Optimal alcohol extraction conditions were as follows： extracted twice with 8 times the amount of 60% ethanol for 1.5h each time; Extracting amount of magnolol was 11.14 mg.g^-1, dry extract rate was 19. 71%. Conclusion： The optimized extraction process was stable and feasible which could provide a reference for rational development of Fufang Baishaoehangkang Granules.