蒸制轮叶党参对顺铂致小鼠肾损伤的保护作用及机制

Protective effect of steamed codonopsis lanceolata on cisplatin-induced kidney damage in mice and its possible mechanism

目的探讨蒸制轮叶党参(steamed codonopsis lanceolata,SCL)对顺铂(Cisplatin)致小鼠肾损伤的保护作用及机制。方法雄性ICR小鼠一次性腹腔注射20 mg/kg顺铂诱发急性肾损伤模型,观察SCL给药后的改善作用。相关试剂盒测定小鼠血清中肌酐(CRE)、尿素氮(BUN)、炎症因子(TNF-α)、白介素1β(IL-1β)和肾组织中丙二醛(MDA)的含量。苏木素-伊红(HE)和Hoechst 33258染色观察肾组织病理学改变。免疫组化法检测肾组织中相关凋亡蛋白的表达。结果与模型组比较,SCL均能不同程度的降低血清中CRE、BUN、TNF-α、IL-1β及组织中MDA的水平(P<0.05),改善顺铂所致的炎症反应,同时SCL能够下调肾组织中Bax,上调Bcl-2的蛋白表达水平。结论 SCL对顺铂致肾损伤具有保护作用,其主要部分通过减少炎症反应,抑制脂质过氧化及抗细胞凋亡而实现的。

Objective To investigate the protective effect of steamed codonopsis lanceolata（ SCL） on cisplatin-induced kidney damage in mice and its possible mechanism. Methods The kidney damage model was established by a single intraperitoneal injection of cisplatin 20 mg / kg in male ICR mice. Blood urinary nitrogen（ BUN）,creatinine（ CRE）,tumor necrosis factor（ TNF-α）,interleukin 1β（ IL-1β） in serum,and malondialdehyde（ MDA） in kidney were determined by relative biochemical kits. HE and Hoechst 33258 staining were employed to evaluate histopathological changes. Immunohistochemistry was used to detect the expression of apoptosis-related protein in kidney tissues. Results Compared with the model group,SCL pretreatment could effectively decrease contents of BUN,CRE,TNF-α,and IL-1β in serum,and MDA in kidney,respectively（ P〈0. 05）. Importantly,immunohistochemical analysis significantly indicated that SCL pretreatment decreased Bax and increased Bcl-2 protein expression levels of kidney tissues in a dose-dependent manner. Conclusion It clearly reveals that the protective effect of SCL on cisplatin-induced kidney damage in mice through inhibiting the lipid peroxidation,decreasing inflammation response,and suppressing tubular cell apoptosis.