Hg-rps-23基因RNAi转基因大豆对大豆胞囊线虫的抗性研究

Generation of Transgenic RNAi Soybean Using Soybean Cyst Nematode Hgrps-23 Gene and Its Resistance to Soybean Cyst Nematode

大豆胞囊线虫（Soybean Cyst Nematode,SCN）是一种极具破坏性的大豆寄生虫,一般可造成大豆减产10%~20%。利用植物介导RNAi是培育抗虫转基因植物新品种的有效手段之一。本研究从大豆胞囊线虫中获得了编码参与线虫m RNA代谢的关键基因-核糖体蛋白基因Hg-rps-23。采用农杆菌介导法将Hg-rps-23 RNA干扰片段导入Williams82中。PCR和Southern杂交检测结果表明,外源RNAi片段已导入受体大豆品种。病土接种鉴定结果表明,转基因大豆对大豆胞囊线虫3号生理小种抗性水平显著提高,表明利用植物介导RNAi可以有效提高大豆抗胞囊线虫水平。本研究为大豆抗胞囊线虫新材料的创制及新品种培育提供了一条新的途径。

Soybean cyst nematode is devastating pathogens that infect soybean and can cause 10% - 20% loss of yield. Plant-host mediated RNAi is one of effective strategy to breeding new insect-resistant transgenic plants, Hgrps-23, which encodes ribosome protein of SCN was cloned into RNAi vector. Then, RNAi construct were transformed into Williams82 by Agrobacterium tumefaciens-mediated transformation. Analysis of transgenic plants using PCR and Southern blot method has proved that the Hg-rps-23 RNAi fragments have inserted into the soybean gehome. T2 transgenic plants were grown in soil with cysts and the results showed that the transgenic plants＇ resistance to SCN3 was significantly higher than that of receptor soybean. Our study suggested that plant-mediated RNAi could enhance the resistance of soybean to SCN. It also provides a new approach to generate SCN-resistant soybean materials and new cultivars.