北虫草生长发育条件的初步探究

A Primary Investigation on Conditions for Growing and Developing of Cordyceps militaris

本文对尖头北虫草(Cordyceps militaris)生长发育条件即:碳源、氮源、无机盐和光质等的最佳组合条件进行探索研究。结果表明:最终筛选出较佳的培养基配方及光质条件。子实体产量方面最佳配方:碳源为葡萄糖5 g/L,氮源为酵母浸膏5 g/L,无机盐为磷酸二氢钾1 g/L,光质为蓝光(或白炽光)。北虫草子实体中合成可溶性糖最多的最佳培养基配方:碳源为可溶性淀粉5 g/L,氮源为蛋白胨5 g/L,无机盐为硫酸镁1 g/L,光质为白炽光;北虫草子实体中合成可溶性蛋白最多的最佳培养基配方:碳源为甘露醇5 g/L,氮源为酵母浸膏5 g/L,无机盐为磷酸二氢钾1 g/L,最佳光质为蓝光。

The optimal conditions for growing and developing conditions of Cordyceps militaris, including carbon source, nitrogen source, mineral salt and light quality, were primary investigated in the paper. The best medium and light quality were selected in the experiment. The best medium for encarpium was that carbon source was 5 g/L glucose, nitrogen source was 5 g/L yeast extract, mineral salt was 1 g/L monopotassium phosphate, and light quality was blue light or incandescence. Besides, the best medium for soluble sugar produced in enearpium of Cordyceps militaris was that carbon source was 5 g/L soluble starch, nitrogen source was 5 g/L peptone, mineral salt was 1 g/L magne- sium sulfate, and light quality was incandescence. On the medium of which carbon source was 5 g/L mannite, nitrogen source was 5g/L yeast extract, mineral salt was 1 g/L monopotassium phosphate, light quality was blue light, more soluble protein in encarpium of Cordyceps militaris could be produced.